Systematic Chemogenetic Library Assembly.

Autor:	Canham SM; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address: steve.canham@novartis.com., Wang Y; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Cornett A; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Auld DS; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address: douglas.auld@novartis.com., Baeschlin DK; Novartis Institute for BioMedical Research, Novartis Pharma AG, Forum 1 Novartis Campus, 4056 Basel, Switzerland., Patoor M; Novartis Institute for BioMedical Research, Novartis Pharma AG, Forum 1 Novartis Campus, 4056 Basel, Switzerland., Skaanderup PR; Novartis Institute for BioMedical Research, Novartis Pharma AG, Forum 1 Novartis Campus, 4056 Basel, Switzerland., Honda A; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Llamas L; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Wendel G; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Mapa FA; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Aspesi P Jr; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Labbé-Giguère N; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Gamber GG; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Palacios DS; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Schuffenhauer A; Novartis Institute for BioMedical Research, Novartis Pharma AG, Forum 1 Novartis Campus, 4056 Basel, Switzerland., Deng Z; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Nigsch F; Novartis Institute for BioMedical Research, Novartis Pharma AG, Forum 1 Novartis Campus, 4056 Basel, Switzerland., Frederiksen M; Novartis Institute for BioMedical Research, Novartis Pharma AG, Forum 1 Novartis Campus, 4056 Basel, Switzerland., Bushell SM; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Rothman D; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Jain RK; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Hemmerle H; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Briner K; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Porter JA; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Tallarico JA; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA., Jenkins JL; Novartis Institute for BioMedical Research, 181 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address: jeremy.jenkins@novartis.com.
Jazyk:	angličtina
Zdroj:	Cell chemical biology [Cell Chem Biol] 2020 Sep 17; Vol. 27 (9), pp. 1124-1129. Date of Electronic Publication: 2020 Jul 23.
DOI:	10.1016/j.chembiol.2020.07.004
Abstrakt:	Chemogenetic libraries, collections of well-defined chemical probes, provide tremendous value to biomedical research but require substantial effort to ensure diversity as well as quality of the contents. We have assembled a chemogenetic library by data mining and crowdsourcing institutional expertise. We are sharing our approach, lessons learned, and disclosing our current collection of 4,185 compounds with their primary annotated gene targets (https://github.com/Novartis/MoaBox). This physical collection is regularly updated and used broadly both within Novartis and in collaboration with external partners. Competing Interests: Declaration of Interests All authors are (or were at the time of their involvement with the construction of the NIBR MoA Box) employees of Novartis. (Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze:	MEDLINE
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