CCL2 and IL18 expressions may associate with the anti-proliferative effect of noncontact electro capacitive cancer therapy in vivo .
| Autor: | Pratiwi R; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.; Graduate School of Biotechnology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Antara NY; Graduate School of Biotechnology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Fadliansyah LG; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Ardiansyah SA; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Nurhidayat L; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Sholikhah EN; Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Sunarti S; Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Widyarini S; Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Fadhlurrahman AG; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Fatmasari H; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Tunjung WAS; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Haryana SM; Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia., Alamsyah F; Center for Medical Physics and Cancer Research, Ctech Labs Edwar Technology, Tangerang, 15320, Indonesia., Taruno WP; Center for Medical Physics and Cancer Research, Ctech Labs Edwar Technology, Tangerang, 15320, Indonesia. |
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| Jazyk: | angličtina |
| Zdroj: | F1000Research [F1000Res] 2019 Oct 17; Vol. 8, pp. 1770. Date of Electronic Publication: 2019 Oct 17 (Print Publication: 2019). |
| DOI: | 10.12688/f1000research.20727.2 |
| Abstrakt: | Background: Noncontact Electro Capacitive Cancer Therapy (ECCT) is a novel treatment modality in cancer. Chemokine (C-C motif) ligand 2 (CCL2) has a major role in the outgrowth of metastatic breast cancer. Interleukin 18 (IL18) plays a role in macrophage alteration, which leads to excessive angiogenesis. This study aims to elaborate on the association of CCL2, IL18, IL23α, and TNF-α (tumor necrosis factor-alpha) expression with the anti-proliferative effect of ECCT in rat breast tumor tissue. Methods: Low intensity (18 Vpp) and intermediate frequency (150 kHz) alternating current-electric field (AC-EF) between two capacitive electrodes were exposed as external EF to a rat cage. Twenty-four rats were divided into four groups of six replicates. Breast tumor tissues were collected from 7, 12-dimethylbenz[a]anthracene (DMBA)-induced rats. Two groups were non DMBA-induced rats without ECCT exposure (NINT) and with (NIT). The other two groups were DMBA-induced rats without ECCT exposure (INT) and with (IT). Mammary glands and breast tumor tissues were collected from each group and preserved. Hematoxylin-eosin and immunohistochemistry staining were performed on paraffin sections of tissues using anti-PCNA, anti-ErbB2, anti-Caspase3, and anti-CD68. CCL2, IL18, IL23α, and TNF-α mRNA relative expressions were analyzed using qRT-PCR. Results: ECCT exposure may cause the reduction of PCNA protein expression as well as ErbB2 on breast tumor tissues, but it causes the increase of Caspase3 and macrophage CD68 protein. In rat breast tumor tissues of IT groups, the mRNA expression of CCL2 and IL18 are significantly down-regulated, in contrast with the up-regulated expression of these cytokines in tumor tissues of the INT group. IL23α and TNF- α expression remained similar in both groups. Conclusion: CCL2 and IL18 expressions have an association with the inhibition of breast tumor cell proliferation affected by ECCT exposure. Competing Interests: No competing interests were disclosed. (Copyright: © 2020 Pratiwi R et al.) |
| Databáze: | MEDLINE |
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