Serum folate and cytokines in heterozygous β-thalassemia.

Autor: Paniz C; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.; Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil., Lucena MR; Disciplina de Hematologia e Hemoterapia, Universidade Federal de São Paulo, São Paulo, Brazil., Bertinato JF; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil., Dos Santos MNN; Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade de Campinas, São Paulo, Brazil., Gomes GW; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil., Figueiredo MS; Disciplina de Hematologia e Hemoterapia, Universidade Federal de São Paulo, São Paulo, Brazil., Sonati MF; Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade de Campinas, São Paulo, Brazil., Blaia-D Avila VLN; Divisão de Hematologia, Faculdade de Medicina e Ciências da Saúde, Pontifícia Universidade Católica de São Paulo, Sorocaba, Brazil., Green R; Department of Pathology and Laboratory Medicine, UC Davis School of Medicine, Sacramento, CA, USA., Guerra-Shinohara EM; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.; Disciplina de Hematologia e Hemoterapia, Universidade Federal de São Paulo, São Paulo, Brazil.; Faculdade e Ciências Farmacêuticas, Alimentos e Nutrição, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
Jazyk: angličtina
Zdroj: International journal of laboratory hematology [Int J Lab Hematol] 2020 Dec; Vol. 42 (6), pp. 718-726. Date of Electronic Publication: 2020 Jul 14.
DOI: 10.1111/ijlh.13287
Abstrakt: Introduction: Folate deficiency is commonly reported in β-thalassemia. Individuals heterozygous for β-thalassemia may have higher folate requirements than normal individuals.
Objectives: To document the concentration of serum total folate and its forms in β-thalassemia heterozygote users (β-TmU) and nonusers (β-TmN) of 5 mg folic acid/d; to determine whether folic acid (FA) consumption from fortified foods allows beta-Tm patients, who do not take FA supplements, to meet their dietary folate requirements; and to investigate the association between higher serum unmetabolized folic acid (UMFA) and inflammatory cytokine concentrations.
Methods: Serum total folate and forms were measured in 42 β-Tm (13 β-TmU and 29 β-TmN) and 84 healthy controls. The mononuclear leucocyte mRNA expression of relevant genes and their products and hematological profiles were determined.
Results: β-TmU had higher serum total folate, 5-methyltetrahydrofolate, UMFA, and tetrahydrofolate (THF) compared with β-TmN. The β-TmN had lower serum total folate and THF than controls. Plasma total homocysteine (tHcy) was lower in β-TmU compared with both β-TmN and controls, while β-TmN had higher tHcy than controls. β-TmU had higher IL-8 than their controls while β-TmN had higher IL-6 and IL-8 than their controls. β-TmU have higher levels of serum total folate, 5- methyltetrahydrofolate, UMFA, and THF than controls. There was no association between UMFA concentrations and cytokine levels.
Conclusions: Mandatory flour fortification with FA in Brazil may be insufficient for β-TmN, since they have higher tHcy and lower serum total folate than controls. Furthermore, β-TmN have higher IL-6 levels than β-TmU. UMFA was not associated with inflammatory cytokine levels.
(© 2020 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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