Evaluation of the sensitivity and specificity of the lateral flow assay, Rose Bengal test and the complement fixation test for the diagnosis of brucellosis in cattle using Bayesian latent class analysis.

Autor: Pfukenyi DM; Department of Clinical Veterinary Studies, Faculty of Veterinary Science, University of Zimbabwe, P.O. Box MP 167, Mount Pleasant, Harare, Zimbabwe., Meletis E; Laboratory of Epidemiology, Biostatistics and Animal Health Economics, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, 224 Trikalon St., Greece., Modise B; Botswana National Veterinary Laboratory, Private Bag 0035, Gaborone, Botswana., Ndengu M; Department of Clinical Veterinary Studies, Faculty of Veterinary Science, University of Zimbabwe, P.O. Box MP 167, Mount Pleasant, Harare, Zimbabwe., Kadzviti FW; Department of Paraclinical Veterinary Studies, Faculty of Veterinary Science, University of Zimbabwe, P.O. Box MP 167, Mount Pleasant, Harare, Zimbabwe., Dipuo K; Botswana National Veterinary Laboratory, Private Bag 0035, Gaborone, Botswana., Moesi K; Botswana National Veterinary Laboratory, Private Bag 0035, Gaborone, Botswana., Kostoulas P; Laboratory of Epidemiology, Biostatistics and Animal Health Economics, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, 224 Trikalon St., Greece., Matope G; Department of Paraclinical Veterinary Studies, Faculty of Veterinary Science, University of Zimbabwe, P.O. Box MP 167, Mount Pleasant, Harare, Zimbabwe. Electronic address: giftmatope@gmail.com.
Jazyk: angličtina
Zdroj: Preventive veterinary medicine [Prev Vet Med] 2020 Aug; Vol. 181, pp. 105075. Date of Electronic Publication: 2020 Jun 21.
DOI: 10.1016/j.prevetmed.2020.105075
Abstrakt: This study was conducted to evaluate the sensitivity (Se) and specificity (Sp) of the Rose Bengal test (RBT), complement fixation test (CFT), the serum lateral flow assay (LFA serum ) and the blood lateral flow assay (LFA blood ) for the detection of antibodies to Brucella spp. using Bayesian latent class models (BLCMs). Sera and whole blood were collected from naturally infected cattle reared in smallholder, small-scale commercial and large-scale commercial farms in Zimbabwe (n = 1022) and Botswana (n = 770). The BLCMs were fitted under the assumption that conditional dependences existed between the tests. Based on the conditional dependence model, the RBT had the highest Se of 0.897 (95 % Probability Intervals: 0.854; 0.932) compared to 0.827 (0.773; 0.872), 0.812 (0.76; 0.858) and 0.809 (0.785; 0.832) for the LFA serum , LFA blood and CFT, respectively. The CFT recorded a higher Sp of 0.999 (0.995; 1.000) than the LFA serum 0.996 (0.99; 1.000), the LFA blood 0.984 (0.976; 0.991) and the RBT 0.969 (0.959; 0.978). The data indicated that both the Se and Sp of RBT and CFT and the Sp of LFA serum and LFA blood were conditionally independent, while the Se appeared to be conditionally dependent. These results indicated that none of the evaluated tests had perfect Se and Sp and consequently could not be used alone for the diagnosis of brucellosis in cattle from the studied farming sectors. Thus, based on high Se and Sp, respectively, a brucellosis testing regimen using the RBT (screening) and the LFA (confirmatory) may be considered.
Competing Interests: Declaration of Competing Interest The authors declare that there are no conflicts of interest.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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