Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina.

Autor: Fusco LS; Laboratorio de Investigación en Proteínas (LabInPro), Instituto de Química Básica y aplicada del Nordeste Argentino (IQUIBA-NEA, CONICET), Universidad Nacional del Nordeste (UNNE), Corrientes, Argentina., Neto EB; Laboratorio de Bioquímica y Biofísica, Instituto Butantan, São Paulo, SP, Brazil., Francisco AF; Departamento de Física e Biofísica, Instituto de Biociências, São Paulo State University, UNESP, Botucatu, SP, Brazil., Alfonso J; Centro de Estudos de Biomoléculas Aplicadas à Saúde (CEBio), Fundação Oswaldo Cruz (FIOCRUZ), Fiocruz Rondônia, Porto Velho-RO e Universidade Federal de Rondônia (UNIR), Porto Velho, RO, Brazil.; Centro Para el Desarrollo de la Investigación Científica (CEDIC), Asunción, Paraguay., Soares A; Centro de Estudos de Biomoléculas Aplicadas à Saúde (CEBio), Fundação Oswaldo Cruz (FIOCRUZ), Fiocruz Rondônia, Porto Velho-RO e Universidade Federal de Rondônia (UNIR), Porto Velho, RO, Brazil.; Centro Universitário São Lucas (UniSL), Porto Velho, RO, Brazil., Pimenta DC; Laboratorio de Bioquímica y Biofísica, Instituto Butantan, São Paulo, SP, Brazil., Leiva LC; Laboratorio de Investigación en Proteínas (LabInPro), Instituto de Química Básica y aplicada del Nordeste Argentino (IQUIBA-NEA, CONICET), Universidad Nacional del Nordeste (UNNE), Corrientes, Argentina.
Jazyk: angličtina
Zdroj: Toxicon: X [Toxicon X] 2020 Jun 15; Vol. 7, pp. 100047. Date of Electronic Publication: 2020 Jun 15 (Print Publication: 2020).
DOI: 10.1016/j.toxcx.2020.100047
Abstrakt: The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus' venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A 2 , serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5'-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2020 Published by Elsevier Ltd.)
Databáze: MEDLINE
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