Human Fallopian Tube Epithelial Cell Culture Model To Study Host Responses to Chlamydia trachomatis Infection.

Autor:	McQueen BE; Department of Pediatrics, University of North Carolina, Chapel Hill, North Carolina, USA.; Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, USA., Kiatthanapaiboon A; Department of Pediatrics, University of North Carolina, Chapel Hill, North Carolina, USA., Fulcher ML; Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, North Carolina, USA., Lam M; Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, North Carolina, USA., Patton K; Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, North Carolina, USA., Powell E; Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, North Carolina, USA., Kollipara A; Department of Pediatrics, University of North Carolina, Chapel Hill, North Carolina, USA.; Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, USA., Madden V; Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA., Suchland RJ; University of Washington, Division of Allergy and Infectious Diseases, Department of Medicine, Seattle, Washington, USA., Wyrick P; Department of Pediatrics, University of North Carolina, Chapel Hill, North Carolina, USA., O'Connell CM; Department of Pediatrics, University of North Carolina, Chapel Hill, North Carolina, USA., Reidel B; Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA., Kesimer M; Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA., Randell SH; Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, North Carolina, USA., Darville T; Department of Pediatrics, University of North Carolina, Chapel Hill, North Carolina, USA.; Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, USA., Nagarajan UM; Department of Pediatrics, University of North Carolina, Chapel Hill, North Carolina, USA nagaraja@email.unc.edu.; Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, USA.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2020 Aug 19; Vol. 88 (9). Date of Electronic Publication: 2020 Aug 19 (Print Publication: 2020).
DOI:	10.1128/IAI.00105-20
Abstrakt:	Chlamydia trachomatis infection of the human fallopian tubes can lead to damaging inflammation and scarring, ultimately resulting in infertility. To study the human cellular responses to chlamydial infection, researchers have frequently used transformed cell lines that can have limited translational relevance. We developed a primary human fallopian tube epithelial cell model based on a method previously established for culture of primary human bronchial epithelial cells. After protease digestion and physical dissociation of excised fallopian tubes, epithelial cell precursors were expanded in growth factor-containing medium. Expanded cells were cryopreserved to generate a biobank of cells from multiple donors and cultured at an air-liquid interface. Culture conditions stimulated cellular differentiation into polarized mucin-secreting and multiciliated cells, recapitulating the architecture of human fallopian tube epithelium. The polarized and differentiated cells were infected with a clinical isolate of C. trachomatis , and inclusions containing chlamydial developmental forms were visualized by fluorescence and electron microscopy. Apical secretions from infected cells contained increased amounts of proteins associated with chlamydial growth and replication, including transferrin receptor protein 1, the amino acid transporters SLC3A2 and SLC1A5, and the T-cell chemoattractants CXCL10, CXCL11, and RANTES. Flow cytometry revealed that chlamydial infection induced cell surface expression of T-cell homing and activation proteins, including ICAM-1, VCAM-1, HLA class I and II, and interferon gamma receptor. This human fallopian tube epithelial cell culture model is an important tool with translational potential for studying cellular responses to Chlamydia and other sexually transmitted pathogens. (Copyright © 2020 American Society for Microbiology.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu