Genetic characterization of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 from diarrhoeic and septicaemic calves and relatedness to human Shigatoxigenic E. coli O80:H2.

Autor: Habets A; Bacteriology, Department of Infectious Diseases, Faculty of Veterinary Medicine, Centre for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège (ULiège), Liège, Belgium., Crombé F; Belgian National Reference Center for STEC (NRC STEC), Universitair Ziekenhuis Brussel (UZ Brussel), Vrije Universiteit Brussel (VUB), Brussels, Belgium., Nakamura K; Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan., Guérin V; Bacteriology, Department of Infectious Diseases, Faculty of Veterinary Medicine, Centre for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège (ULiège), Liège, Belgium., De Rauw K; Belgian National Reference Center for STEC (NRC STEC), Universitair Ziekenhuis Brussel (UZ Brussel), Vrije Universiteit Brussel (VUB), Brussels, Belgium., Piérard D; Belgian National Reference Center for STEC (NRC STEC), Universitair Ziekenhuis Brussel (UZ Brussel), Vrije Universiteit Brussel (VUB), Brussels, Belgium., Saulmont M; Association Régionale de Santé et d'Identification Animale (ARSIA), Ciney, Belgium., Hayashi T; Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan., Mainil JG; Bacteriology, Department of Infectious Diseases, Faculty of Veterinary Medicine, Centre for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège (ULiège), Liège, Belgium., Thiry D; Bacteriology, Department of Infectious Diseases, Faculty of Veterinary Medicine, Centre for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège (ULiège), Liège, Belgium.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2021 Jan; Vol. 130 (1), pp. 258-264. Date of Electronic Publication: 2020 Jul 16.
DOI: 10.1111/jam.14759
Abstrakt: Aim: The purpose of this work was to identify and genetically characterize enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) O80:H2 from diarrhoeic and septicaemic calves in Belgium and to comparing them with human EHEC after whole genome sequencing.
Methods and Results: Ten EHEC and 21 EPEC O80 identified by PCR between 2009 and 2018 from faeces, intestinal content and a kidney of diarrhoeic or septicaemic calves were genome sequenced and compared to 19 human EHEC identified between 2008 and 2019. They all belonged to the O80:H2 serotype and ST301, harboured the eaeξ gene, and 23 of the 29 EHEC contained the stx2d gene. Phylogenetically, they were distributed in two major sub-lineages: one comprised a majority of bovine EPEC whereas the second one comprised a majority of stx2d bovine and human EHEC.
Conclusions: Not only EPEC but also EHEC O80:H2 are present in diarrhoeic and septicaemic calves in Belgium and are genetically related to human EHEC.
Significance and Impact of the Study: These findings support the need to assess cattle as potential source of contamination of humans by EHEC O80:H2 and to understand the evolution of bovine and human EHEC and EPEC O80:H2.
(© 2020 The Society for Applied Microbiology.)
Databáze: MEDLINE
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