Investigation of the gut microbiome of Apis cerana honeybees from Vietnam.

Autor: Duong BTT; Molecular Microbiology Lab, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam., Lien NTK; Functional Genomics Lab, Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam., Thu HT; Molecular Microbiology Lab, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam., Hoa NT; Molecular Microbiology Lab, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam., Lanh PT; Molecular Microbiology Lab, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam., Yun BR; Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, 39660, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea., Yoo MS; Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, 39660, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea., Cho YS; Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, 39660, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea. choys@korea.kr., Van Quyen D; Molecular Microbiology Lab, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam. dvquyen@gmail.com.; University of Science and Technology of Ha Noi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam. dvquyen@gmail.com.
Jazyk: angličtina
Zdroj: Biotechnology letters [Biotechnol Lett] 2020 Nov; Vol. 42 (11), pp. 2309-2317. Date of Electronic Publication: 2020 Jun 23.
DOI: 10.1007/s10529-020-02948-4
Abstrakt: Objectives: In this study, the gut microbiome of healthy adult honeybees, Apis cerana, was investigated by sequencing the V3 - V4 region in 16S rRNA gene using Illumina Miseq platform.
Results: The total of 37,853 reads for 16S rRNA gene were obtained and 30,121 (79.6%) reads were valid with 25,291 (84.0%) reads that were classified into 116 species belonging to four major phyla. The relative abundances of the bacterial isolates in honeybee samples were phylum Proteobacteria (70.7%), Actinobacteria (10.7%), Firmicutes (10.3%), and Bacteroidetes (8.4%), respectively. Lactic acid bacteria comprised 18.95% with 10 groups including Bifidobacterium asteroides, B. indicum, Fructobacillus fructosus, Lactobacillus apinorum, L. apis, L. helsingborgensis, L. kimbladii, L. kullabergensis, and L. kunkeei.
Conclusions: The presence of beneficial bacteria in the gut highlighted their role in the honeybee and suggested that they can be promising candidates for the development of probiotics for health improvement, infection control and disease management of honeybees.
Databáze: MEDLINE
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