| Autor: |
Brozek CM; Lovelace Medical Foundation, Biomedical Research Division, Albuquerque, New Mexico 87108., Hoffman CL, Savage SM, Searles RP |
| Jazyk: |
angličtina |
| Zdroj: |
Clinical immunology and immunopathology [Clin Immunol Immunopathol] 1988 Feb; Vol. 46 (2), pp. 299-313. |
| DOI: |
10.1016/0090-1229(88)90192-4 |
| Abstrakt: |
Several reports have demonstrated that systemic lupus erythematosus (SLE) patients have a decreased response to exogenous antigens both in vivo and in vitro. We examined the effects of SLE sera on macrophage (M phi) antigen-presenting functions. M phi from normal donors were pulsed with tetanus toxoid antigen in the presence of SLE or normal human serum (NHS), fixed in paraformaldehyde, and incubated with autologous T cells. Of 16 SLE sera tested, 11 inhibited the T-cell proliferative response (measured by [3H]thymidine uptake) compared to control NHS; mean percentage inhibition was 53 +/- 23%. This inhibition did not result from interference with antigen uptake by M phi and was found in both IgM and IgG fractions of the sera. There was a positive correlation between the amount of inhibition and the cytotoxic reactivity of the SLE sera against M phi as measured by Terasaki assay (r = 0.659, P less than 0.01). However, the presence and the amount of the inhibition did not correlate with serum immune complexes by Clq ELISA, serum anti-DR antibodies, or clinical disease activity of the SLE patients. We conclude that some SLE sera possess IgM and IgG antibodies reactive with M phi which affect M phi antigen-presenting functions, and might relate to decreased antigenic response in SLE patients. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
