Immunohistochemical expression of Cyclin D1 among Sudanese patients diagnosed with benign and malignant prostatic lesions.

Autor: Ahmed ES; Mycetoma Research Center, University of Khartoum, Khartoum, Sudan., Elnour LS; Department of Cytology and Histopathology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan., Hassan R; Mycetoma Research Center, University of Khartoum, Khartoum, Sudan., Siddig EE; Mycetoma Research Center, University of Khartoum, Khartoum, Sudan.; Department of Cytology and Histopathology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.; Nile University- School of Medicine, Khartoum, Sudan.; Department of Histopathology and Cytology, Alfarrabi College for Science and Technology, Khartoum, Sudan., Chacko ME; Faculty of Medicine, National University, Khartoum, Sudan., Ali ET; Department of Cytology and Histopathology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.; Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, National University, Khartoum, Sudan., Mohamed MA; Department of Parasitology and Medical Entomology, Faculty of Medical Laboratory Sciences, Nile University, Khartoum, Sudan., Munir A; Department of Bioinformatics and Biostatistics, National University Research Institute, National University, Khartoum, Sudan., Muneer MS; Department of Neurology, Mayo Clinic, Jacksonville, FL, USA.; Department of Radiology, Mayo Clinic, Jacksonville, FL, USA.; Department of Internal Medicine, Faculty of Medicine, University of Khartoum, Khartoum, Sudan., Mohamed NS; Department of Parasitology and Medical Entomology, Faculty of Medical Laboratory Sciences, Nile University, Khartoum, Sudan. nouh_saad@outlook.com.; Department of Parasitology and Medical Entomology, Faculty of Medicine, Sinnar University, Sinnar, Sudan. nouh_saad@outlook.com.; Molecular Biology Department, Alfarrabi College for sciences and Technology, Khartoum, Sudan. nouh_saad@outlook.com., Edris AMM; Department of Cytology and Histopathology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.; Department of Histopathology and Cytology, Faculty of Applied Medical Sciences, University of Bisha, Bisha, Kingdom of Saudi Arabia.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2020 Jun 17; Vol. 13 (1), pp. 295. Date of Electronic Publication: 2020 Jun 17.
DOI: 10.1186/s13104-020-05138-7
Abstrakt: Objectives: Prostate cancer (PC) is common cancer worldwide. Several markers have been developed to differentiate between benign prostatic hyperplasia (BPH) from PC. A descriptive retrospective hospital-based study aimed at determining the expression of Cyclin D1 in BPH and PC. The study took place at different histopathology laboratories in Khartoum state, Sudan, from December 2016 to January 2019. Formalin-fixed paraffin-embedded blocks were sectioned and fixed in 3-aminopropyltriethoxysilane coated slides incubated into primary antibody for Cyclin D1. The assessment of immunoreactivity of Cyclin D1 of each section was done using the Gleason scoring system.
Results: A total of 153 males' prostate sections included in this study, of them, 120 (78.4%) were PC, and 33 (21.6%) were BPH. Their age ranged from 45 to 88 years, mean age was 66.19 ± 8.599. 142 (92.8%) did not have a family history of PC, while 11 (7.2%) patients reported having a family history. The Gleason scoring showed a total of 81 (52.9%) patients with high-grade and 39 (25.5%) with low-grade. 118 (97.5%) patients had PC showed positive results for Cyclin D1, while BPH was 3 (2.5%). P value < 0.001. Cyclin D1 staining was associated with high-grade Gleason score and perineural invasion, P value 0.001.
Databáze: MEDLINE
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