Use of MALDI-TOF MS (Bruker Daltonics) for identification of Mycobacterium species isolated directly from liquid medium.

Autor: Fernández-Esgueva M; Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón, Zaragoza, Spain. Electronic address: martafdzesg@hotmail.com., Fernández-Simon R; Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón, Zaragoza, Spain., Monforte-Cirac ML; Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón, Zaragoza, Spain., López-Calleja AI; Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón, Zaragoza, Spain., Fortuño B; Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón, Zaragoza, Spain., Viñuelas-Bayon J; Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón, Zaragoza, Spain; Grupo de estudio de las Infecciones por Micobacterias (GEIM), Sociedad Española de Microbiología y Enfermedades Infecciosas, Spain.
Jazyk: English; Spanish; Castilian
Zdroj: Enfermedades infecciosas y microbiologia clinica (English ed.) [Enferm Infecc Microbiol Clin (Engl Ed)] 2021 May; Vol. 39 (5), pp. 241-243. Date of Electronic Publication: 2020 Jun 12.
DOI: 10.1016/j.eimc.2020.05.011
Abstrakt: Objectives: The aim of this study was to describe the evaluation of the use of MALDI-TOF MS for the identification of non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis directly from liquid MGIT cultures from January 2017 to December 2017.
Material/methods: A total of 155 isolates (mainly respiratory) were analyzed by MALDI-TOF MS (Bruker Daltonics) directly from MGIT liquid medium with a previous extraction procedure.
Results: MALDI-TOF MS generated acceptable scores for 152 isolates (98.06%). Fifty isolates were identified as M. tuberculosis complex and the remaining 105 as NTM (M. abscessus subsp. abscessus, M. avium, M. celatum, M chelonae, M. chimaera, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. lentiflavum, M. mageritense, M. mucogenicum and M. xenopi).
Conclusions: These results indicate that MALDI-TOF MS can be useful to identify mycobacteria directly from MGIT cultures and is an accurate, rapid and cost-effective system to be used as a routine method.
(Copyright © 2020 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.)
Databáze: MEDLINE
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