A quantitative serum biomarker of circulating collagen X effectively correlates with endochondral fracture healing.

Autor: Working ZM; Department of Orthopaedic Surgery, Orthopaedic Trauma Institute, Zuckerberg San Francisco General Hospital (ZSFG), University of California, San Francisco (UCSF), San Francisco, California.; Orthopaedics and Rehabilitation, Oregon Health & Science University (OHSU), Portland, Oregon., Morris ER; Center for Regenerative Sports Medicine, Steadman Philippon Research Institute (SPRI), Vail, Colorado., Chang JC; Department of Orthopaedic Surgery, Orthopaedic Trauma Institute, Zuckerberg San Francisco General Hospital (ZSFG), University of California, San Francisco (UCSF), San Francisco, California., Coghlan RF; Shriners Hospitals for Children, Research Center, Portland, Oregon., Johnstone B; Orthopaedics and Rehabilitation, Oregon Health & Science University (OHSU), Portland, Oregon., Miclau T 3rd; Department of Orthopaedic Surgery, Orthopaedic Trauma Institute, Zuckerberg San Francisco General Hospital (ZSFG), University of California, San Francisco (UCSF), San Francisco, California., Horton WA; Shriners Hospitals for Children, Research Center, Portland, Oregon., Bahney CS; Department of Orthopaedic Surgery, Orthopaedic Trauma Institute, Zuckerberg San Francisco General Hospital (ZSFG), University of California, San Francisco (UCSF), San Francisco, California.; Center for Regenerative Sports Medicine, Steadman Philippon Research Institute (SPRI), Vail, Colorado.
Jazyk: angličtina
Zdroj: Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2021 Jan; Vol. 39 (1), pp. 53-62. Date of Electronic Publication: 2020 Jul 20.
DOI: 10.1002/jor.24776
Abstrakt: Currently, there are no standardized methods for quantitatively measuring fracture repair. Physicians rely on subjective physical examinations and qualitative evaluation of radiographs to detect mineralized tissue. Since most fractures heal indirectly through a cartilage intermediate, these tools are limited in their diagnostic utility of early repair. Prior to converting to the bone, cartilage undergoes hypertrophic maturation, characterized by the deposition of a provisional collagen X matrix. The objective of this study was to characterize the kinetics of a novel collagen X biomarker relative to other biological measurements of fracture healing using a murine model of endochondral fracture repair in which a closed, mid-shaft tibia fracture was created using the classic drop-weight technique. Serum was collected 5 to 42 days post-fracture in male and female mice and compared to uninjured controls (n = 8-12). Collagen X in the serum was quantified using a recently validated ELISA-based bioassay ("Cxm") 1 and compared to genetic and histological markers of fracture healing and inflammation. We found the Cxm biomarker reliably increased from baseline to a statistically unique peak 14 days post-fracture that then resolved to pre-fracture levels by 3 weeks following injury. The shape and timing of the Cxm curve followed the genetic and histological expression of collagen X but did not show a strong correlation with local inflammatory states. Assessment of fracture healing progress is crucial to making correct and timely clinical decisions for patients. This Cxm bioassay represents a minimally invasive, inexpensive technique that could provide reliable information on the biology of the fracture to significantly improve clinical care.
(© 2020 Orthopaedic Research Society. Published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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