| Autor: |
Durán-Meza AL; Biological Physics Laboratory, Universidad Autónoma de San Luis Potosí, Álvaro Obregón 64, San Luis Potosí 78000, Mexico., Escamilla-Ruiz MI; Biological Physics Laboratory, Universidad Autónoma de San Luis Potosí, Álvaro Obregón 64, San Luis Potosí 78000, Mexico., Segovia-González XF; Biological Physics Laboratory, Universidad Autónoma de San Luis Potosí, Álvaro Obregón 64, San Luis Potosí 78000, Mexico., Villagrana-Escareño MV; Biological Physics Laboratory, Universidad Autónoma de San Luis Potosí, Álvaro Obregón 64, San Luis Potosí 78000, Mexico., Vega-Acosta JR; Biological Physics Laboratory, Universidad Autónoma de San Luis Potosí, Álvaro Obregón 64, San Luis Potosí 78000, Mexico., Ruiz-Garcia J; Biological Physics Laboratory, Universidad Autónoma de San Luis Potosí, Álvaro Obregón 64, San Luis Potosí 78000, Mexico. |
| Jazyk: |
angličtina |
| Zdroj: |
Molecules (Basel, Switzerland) [Molecules] 2020 Jun 05; Vol. 25 (11). Date of Electronic Publication: 2020 Jun 05. |
| DOI: |
10.3390/molecules25112628 |
| Abstrakt: |
Different types of gold nanoparticles have been synthesized that show great potential in medical applications such as medical imaging, bio-analytical sensing and photothermal cancer therapy. However, their stability, polydispersity and biocompatibility are major issues of concern. For example, the synthesis of gold nanorods, obtained through the elongated micelle process, produce them with a high positive surface charge that is cytotoxic, while gold nanoshells are unstable and break down in a few weeks due to the Ostwald ripening process. In this work, we report the self-assembly of the capsid protein (CP) of cowpea chlorotic mottle virus (CCMV) around spherical gold nanoparticles, gold nanorods and gold nanoshells to form virus-like particles (VLPs). All gold nanoparticles were synthesized or treated to give them a negative surface charge, so they can interact with the positive N-terminus of the CP leading to the formation of the VLPs. To induce the protein self-assembly around the negative gold nanoparticles, we use different pH and ionic strength conditions determined from a CP phase diagram. The encapsidation with the viral CP will provide the nanoparticles better biocompatibility, stability, monodispersity and a new biological substrate on which can be introduced ligands toward specific cells, broadening the possibilities for medical applications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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