Human immunoglobulin G responses to Cimex lectularius L. saliva.

Autor: Sheele JM; Department of Emergency Medicine, Mayo Clinic, Jacksonville, Florida, USA., Ferrari B; Immune Function Core Facility, Case Western Reserve University, Cleveland, Ohio, USA., Goddard J; Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Starkville, Mississippi, USA., Schlatzer D; Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, Ohio, USA., Lundberg KC; Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, Ohio, USA., Guinto K; Case Western Reserve University, Cleveland, Ohio, USA., Embers ME; Division of Immunology, Tulane University National Primate Research Center, Covington, Louisiana, USA., Young AB; Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA., Ridge GE; The Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA., Damiani G; Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA., McCormick TS; Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
Jazyk: angličtina
Zdroj: Parasite immunology [Parasite Immunol] 2020 Dec; Vol. 42 (12), pp. e12764. Date of Electronic Publication: 2020 Jun 22.
DOI: 10.1111/pim.12764
Abstrakt: Aims: To investigate the immunoglobulin (Ig) G response after being fed upon by Cimex lectularius L.
Methods and Results: Participants were fed upon by three male C lectularius insects weekly for a month. Blood was obtained before the feeding and at the last feeding, which was used for immunoblots against bed bug salivary gland extract, with antihuman Immunoglobulin G (IgG) secondary antibodies. No consistent IgG changes developed in 11 humans serially fed upon by C lectularius. Two participants had new IgG responses to proteins at molecular weights of approximately 12-13 kDa, and one had an IgG response to a protein at approximately 40 kDa. At the last study visit, more intense IgG bands to proteins at molecular weights of 12-13 kDa had developed in 55% of participants (6/11) and at molecular weights of ≈30, ≈40 and ≈70 kDa in 45% (5/11) compared with the first study visit. Nitrophorin and apyrase were the most common C lectularius proteins identified with liquid chromatography-tandem mass spectrometry in both crushed bed bug salivary gland extract and post-bed bug feeding extract.
Conclusions: Human participants did not have consistent IgG responses to crushed C lectularius salivary gland extract.
(© 2020 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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