Analytical methods in strigolactone research.

Autor: Halouzka R; Centre of Region Haná for Biotechnological and Agricultural Research, Department of Phytochemistry, Faculty of Science, Palacky University, Šlechtitelů 27, 78371 Olomouc, Czechia., Zeljković SĆ; Centre of Region Haná for Biotechnological and Agricultural Research, Department of Phytochemistry, Faculty of Science, Palacky University, Šlechtitelů 27, 78371 Olomouc, Czechia.; Centre of Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Šlechtitelů 29, 78371 Olomouc, Czechia., Klejdus B; Central European Institute of Technology, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czechia.; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czechia., Tarkowski P; Centre of Region Haná for Biotechnological and Agricultural Research, Department of Phytochemistry, Faculty of Science, Palacky University, Šlechtitelů 27, 78371 Olomouc, Czechia.; Centre of Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Šlechtitelů 29, 78371 Olomouc, Czechia.
Jazyk: angličtina
Zdroj: Plant methods [Plant Methods] 2020 May 29; Vol. 16, pp. 76. Date of Electronic Publication: 2020 May 29 (Print Publication: 2020).
DOI: 10.1186/s13007-020-00616-2
Abstrakt: Strigolactones (SLs) are important plant hormones that are produced via the carotenoid biosynthetic pathway and occur at extremely low concentrations in various plant species. They regulate root development, play important roles in symbioses between higher plants and mycorrhizal fungi, and stimulate germination of plant-parasitic Orobanche and Striga species. Chemical analysis is central to research on the biochemistry of SLs and their roles in developmental biology and plant physiology. Here we summarize key issues relating to the identification and quantification of SLs isolated from plant tissues and exudates. The advantages and drawbacks of different protocols used for strigolactone analysis are discussed, and guidelines for selecting a procedure that will minimize losses during isolation and purification prior to final analysis are proposed. Hyphenated techniques suitable for SL analysis such as GC-MS and LC-MS/MS are also discussed, and newer ambient techniques such as HR-DART-MS and DESI-MS are highlighted as tools with considerable potential in SL research. A key advantage of these methods is that they require only simply sample preparation.
Competing Interests: Competing interestsThe authors declared that they have no competing interests.
(© The Author(s) 2020.)
Databáze: MEDLINE
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