Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses.

Autor:	Goncalves J; Interdisciplinary Institute for Neuroscience, University of Bordeaux, 33077 Bordeaux, France.; Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, 33077 Bordeaux, France., Bartol TM; Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037., Camus C; Interdisciplinary Institute for Neuroscience, University of Bordeaux, 33077 Bordeaux, France.; Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, 33077 Bordeaux, France., Levet F; Interdisciplinary Institute for Neuroscience, University of Bordeaux, 33077 Bordeaux, France.; Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, 33077 Bordeaux, France.; Bordeaux Imaging Center, CNRS UMS 3420, University of Bordeaux, INSERM US04, 33077 Bordeaux, France., Menegolla AP; Interdisciplinary Institute for Neuroscience, University of Bordeaux, 33077 Bordeaux, France.; Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, 33077 Bordeaux, France., Sejnowski TJ; Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037., Sibarita JB; Interdisciplinary Institute for Neuroscience, University of Bordeaux, 33077 Bordeaux, France.; Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, 33077 Bordeaux, France., Vivaudou M; Institut de Biologie Structurale, Université Grenoble Alpes, Commissariat à l'Énergie Atomique, CNRS, 38000 Grenoble, France.; Laboratory of Excellence, Ion Channel Science and Therapeutics, 06560 Nice, France., Choquet D; Interdisciplinary Institute for Neuroscience, University of Bordeaux, 33077 Bordeaux, France.; Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, 33077 Bordeaux, France.; Bordeaux Imaging Center, CNRS UMS 3420, University of Bordeaux, INSERM US04, 33077 Bordeaux, France., Hosy E; Interdisciplinary Institute for Neuroscience, University of Bordeaux, 33077 Bordeaux, France; eric.hosy@u-bordeaux.fr.; Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, 33077 Bordeaux, France.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2020 Jun 23; Vol. 117 (25), pp. 14503-14511. Date of Electronic Publication: 2020 Jun 08.
DOI:	10.1073/pnas.1922563117
Abstrakt:	The nanoscale co-organization of neurotransmitter receptors facing presynaptic release sites is a fundamental determinant of their coactivation and of synaptic physiology. At excitatory synapses, how endogenous AMPARs, NMDARs, and mGluRs are co-organized inside the synapse and their respective activation during glutamate release are still unclear. Combining single-molecule superresolution microscopy, electrophysiology, and modeling, we determined the average quantity of each glutamate receptor type, their nanoscale organization, and their respective activation. We observed that NMDARs form a unique cluster mainly at the center of the PSD, while AMPARs segregate in clusters surrounding the NMDARs. mGluR5 presents a different organization and is homogenously dispersed at the synaptic surface. From these results, we build a model predicting the synaptic transmission properties of a unitary synapse, allowing better understanding of synaptic physiology. Competing Interests: The authors declare no competing interest.
Databáze:	MEDLINE
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