Autonomous synthesis and assembly of a ribosomal subunit on a chip.
| Autor: | Levy M; Department of Chemical and Biological Physics Weizmann Institute of Science, Rehovot 7610001, Israel., Falkovich R; Department of Chemical and Biological Physics Weizmann Institute of Science, Rehovot 7610001, Israel., Daube SS; Department of Chemical and Biological Physics Weizmann Institute of Science, Rehovot 7610001, Israel., Bar-Ziv RH; Department of Chemical and Biological Physics Weizmann Institute of Science, Rehovot 7610001, Israel. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Science advances [Sci Adv] 2020 Apr 15; Vol. 6 (16), pp. eaaz6020. Date of Electronic Publication: 2020 Apr 15 (Print Publication: 2020). |
| DOI: | 10.1126/sciadv.aaz6020 |
| Abstrakt: | Ribosome biogenesis is an efficient and complex assembly process that has not been reconstructed outside a living cell so far, yet is the most critical step for establishing a self-replicating artificial cell. We recreated the biogenesis of Escherichia coli's small ribosomal subunit by synthesizing and capturing all its ribosomal proteins and RNA on a chip. Surface confinement provided favorable conditions for autonomous stepwise assembly of new subunits, spatially segregated from original intact ribosomes. Our real-time fluorescence measurements revealed hierarchal assembly, cooperative interactions, unstable intermediates, and specific binding to large ribosomal subunits. Using only synthetic genes, our methodology is a crucial step toward creation of a self-replicating artificial cell and a general strategy for the mechanistic investigation of diverse multicomponent macromolecular machines. (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|