| Autor: |
Aslam Khan MU; School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University (SJTU), 1954 Huashan Road, Shanghai 200030, China.; School of Biomedical Engineering and Health Sciences, Universiti Teknologi Malaysia, Skudai 81300, Johor, Malaysia., Mehboob H; Department of Engineering Management, College of Engineering, Prince Sultan University, P.O. Box No. 66833, Rafha Street, Riyadh 11586, Saudi Arabia., Abd Razak SI; School of Biomedical Engineering and Health Sciences, Universiti Teknologi Malaysia, Skudai 81300, Johor, Malaysia.; Centre for Advanced Composite Materials, Universiti Teknologi Malaysia, Skudai 81300, Johor, Malaysia., Yahya MY; Centre for Advanced Composite Materials, Universiti Teknologi Malaysia, Skudai 81300, Johor, Malaysia., Mohd Yusof AH; School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai 81300, Johor, Malaysia., Ramlee MH; Medical Devices and Technology Centre (MEDiTEC), Institute of Human-Centered Engineering (iHumEn), Universiti Teknologi Malaysia, Skudai 81300, Johor, Malaysia., Sahaya Anand TJ; Sustainable and Responsive Manufacturing Group, Fakulti Kejuruteraan Pembuatan, Universiti Teknikal Malaysia Melaka, Hang Tuah Jaya, Durian Tunggal 76100, Melaka, Malaysia., Hassan R; Orthodontic Unit, School of Dental Science, Universiti Sains Malaysia, Kelantan 16150, Malaysia., Aziz A; School of Environment and Life Sciences, Biomedical Research Centre University of Salford, Manchester M5 4WT, UK., Amin R; Department of Biology, College of Sciences, University of Hafr Al Batin, Hafar Al-Batin 39524, Saudi Arabia. |
| Abstrakt: |
Advancement and innovation in bone regeneration, specifically polymeric composite scaffolds, are of high significance for the treatment of bone defects. Xyloglucan (XG) is a polysaccharide biopolymer having a wide variety of regenerative tissue therapeutic applications due to its biocompatibility, in-vitro degradation and cytocompatibility. Current research is focused on the fabrication of polymeric bioactive scaffolds by freeze drying method for nanocomposite materials. The nanocomposite materials have been synthesized from free radical polymerization using n-SiO 2 and n-HAp XG and Methacrylic acid (MAAc). Functional group analysis, crystallinity and surface morphology were investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD) and scanning electron microscopy (SEM) techniques, respectively. These bioactive polymeric scaffolds presented interconnected and well-organized porous morphology, controlled precisely by substantial ratios of n-SiO 2 . The swelling analysis was also performed in different media at varying temperatures (27, 37 and 47 °C) and the mechanical behavior of the dried scaffolds is also investigated. Antibacterial activities of these scaffolds were conducted against pathogenic gram-positive and gram-negative bacteria. Besides, the biological behavior of these scaffolds was evaluated by the Neutral Red dye assay against the MC3T3-E1 cell line. The scaffolds showed interesting properties for bone tissue engineering, including porosity with substantial mechanical strength, biodegradability, biocompatibility and cytocompatibility behavior. The reported polymeric bioactive scaffolds can be aspirant biomaterials for bone tissue engineering to regenerate defecated bone. |
