Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs.

Autor: Ellison EE; Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul, MN, USA.; Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN, USA.; Center for Genome Engineering, University of Minnesota, St. Paul, MN, USA.; Plant and Microbial Biology Graduate Program, University of Minnesota, St. Paul, MN, USA., Nagalakshmi U; Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis, Davis, CA, USA., Gamo ME; Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul, MN, USA.; Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN, USA.; Center for Genome Engineering, University of Minnesota, St. Paul, MN, USA., Huang PJ; Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis, Davis, CA, USA., Dinesh-Kumar S; Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis, Davis, CA, USA., Voytas DF; Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul, MN, USA. voytas@umn.edu.; Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN, USA. voytas@umn.edu.; Center for Genome Engineering, University of Minnesota, St. Paul, MN, USA. voytas@umn.edu.
Jazyk: angličtina
Zdroj: Nature plants [Nat Plants] 2020 Jun; Vol. 6 (6), pp. 620-624. Date of Electronic Publication: 2020 Jun 01.
DOI: 10.1038/s41477-020-0670-y
Abstrakt: An in planta gene editing approach was developed wherein Cas9 transgenic plants are infected with an RNA virus that expresses single guide RNAs (sgRNAs). The sgRNAs are augmented with sequences that promote cell-to-cell mobility. Mutant progeny are recovered in the next generation at frequencies ranging from 65 to 100%; up to 30% of progeny derived from plants infected with a virus expressing three sgRNAs have mutations in all three targeted loci.
Databáze: MEDLINE
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