| Autor: |
Koop AC; Center for Congenital Heart Diseases, Department of Pediatric Cardiology, University Medical Center Groningen, University of Groningen; a.c.koop@umcg.nl., Hagdorn QAJ; Center for Congenital Heart Diseases, Department of Pediatric Cardiology, University Medical Center Groningen, University of Groningen., van de Kolk KCWA; The Central Animal Facility, University Medical Center Groningen, University of Groningen; Gronsai (Groningen Small Animal Imaging Facility), University Medical Center Groningen, University of Groningen., van Oosten A; The Central Animal Facility, University Medical Center Groningen, University of Groningen., Weij M; The Central Animal Facility, University Medical Center Groningen, University of Groningen., Silljé HHW; Department of Cardiology, University Medical Center Groningen, University of Groningen., Willems TP; Department of Radiology, University Medical Center Groningen, University of Groningen., Berger RMF; Center for Congenital Heart Diseases, Department of Pediatric Cardiology, University Medical Center Groningen, University of Groningen. |
| Abstrakt: |
Right ventricular (RV) function and failure are major determinants of outcome in acquired and congenital heart diseases, including pulmonary hypertension. Assessment of RV function and morphology is complex, partly due to the complex shape of the RV. Currently, cardiac magnetic resonance (CMR) imaging is the golden standard for noninvasive assessment of RV function and morphology. The current protocol describes CMR imaging in a mouse model of RV pressure load induced by pulmonary artery banding (PAB). PAB is performed by placing a 6-0 suture around the pulmonary artery over a 23 G needle. The PAB gradient is determined using echocardiography at 2 and 6 weeks. At 6 weeks, the right and left ventricular morphology and function is assessed by measuring both end-systolic and end-diastolic volumes and mass by ten to eleven cine slices 1 mm thick using a 9.4 T magnetic resonance imaging scanner equipped with a 1,500 mT/m gradient. Representative results show that PAB induces a significant increase in RV pressure load, with significant effects on biventricular morphology and RV function. It is also shown that at 6 weeks of RV pressure load, cardiac output is maintained. Presented here is a reproducible protocol for the quantification of biventricular morphology and function in a mouse model of RV pressure load and may serve as a method for experiments exploring determinants of RV remodeling and dysfunction. |
