| Autor: |
Ripoll C; Departamento de Fisicoquimica, Facultad de Farmacia, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Campus Cartuja, 18071 Granada, Spain., Roldan M; GENYO, Pfizer-Universidad de Granada-Junta de Andalucia Centre for Genomics and Oncological Research, Avda Ilustracion 114, PTS, 18016 Granada, Spain., Contreras-Montoya R; Departamento de Quimica Orgánica, Facultad de Ciencias, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Campus Fuentenueva, 18071 Granada, Spain., Diaz-Mochon JJ; GENYO, Pfizer-Universidad de Granada-Junta de Andalucia Centre for Genomics and Oncological Research, Avda Ilustracion 114, PTS, 18016 Granada, Spain.; Departamento de Quimica Farmaceutica y Orgánica, Facultad de Farmacia, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Campus Cartuja, 18071 Granada, Spain., Martin M; GENYO, Pfizer-Universidad de Granada-Junta de Andalucia Centre for Genomics and Oncological Research, Avda Ilustracion 114, PTS, 18016 Granada, Spain.; Departamento de Bioquimica y Biologia Celular I, Facultad de Ciencias, Universidad de Granada, Campus Fuentenueva, 18071 Granada, Spain., Ruedas-Rama MJ; Departamento de Fisicoquimica, Facultad de Farmacia, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Campus Cartuja, 18071 Granada, Spain., Orte A; Departamento de Fisicoquimica, Facultad de Farmacia, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Campus Cartuja, 18071 Granada, Spain. |
| Abstrakt: |
The main role of mitochondria, as pivotal organelles for cellular metabolism, is the production of energy (ATP) through an oxidative phosphorylation system. During this process, the electron transport chain creates a proton gradient that drives the synthesis of ATP. One of the main features of tumoral cells is their altered metabolism, providing alternative routes to enhance proliferation and survival. Hence, it is of utmost importance to understand the relationship between mitochondrial pH, tumoral metabolism, and cancer. In this manuscript, we develop a highly specific nanosensor to accurately measure the intramitochondrial pH using fluorescence lifetime imaging microscopy (FLIM). Importantly, we have applied this nanosensor to establish differences that may be hallmarks of different metabolic pathways in breast cancer cell models, leading to the characterization of different metabophenotypes. |
