| Autor: |
Alvarenga MOP; Laboratory of Functional and Structural Biology, Biological Sciences Institute, Federal University of Pará, Belém 66075-110, Brazil., Bittencourt LO; Laboratory of Functional and Structural Biology, Biological Sciences Institute, Federal University of Pará, Belém 66075-110, Brazil., Mendes PFS; Laboratory of Functional and Structural Biology, Biological Sciences Institute, Federal University of Pará, Belém 66075-110, Brazil., Ribeiro JT; Faculty of Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 90040-060, Brazil., Lameira OA; Laboratory of Biotechnology, Embrapa Amazônia Oriental, Belém 66075-110, Brazil., Monteiro MC; Laboratory of In Vitro Tests, Immunology and Microbiology, Health Science Institute, Federal University of Pará, Belém 66075-110, Brazil., Barboza CAG; Department of Morphology, Federal University of Rio Grande do Norte, Natal 59078-900, Brazil., Martins MD; Faculty of Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 90040-060, Brazil., Lima RR; Laboratory of Functional and Structural Biology, Biological Sciences Institute, Federal University of Pará, Belém 66075-110, Brazil. |
| Abstrakt: |
In traditional communities of the Brazilian Amazon, the copaiba oleoresin ( C. reticulata Ducke) is widely known for its therapeutic activity, especially its wound healing and anti-inflammatory actions. Our study aimed to evaluate these effects in oral lesions and the safety of the dosage proposed. A punch biopsy wound was induced on the ventral surface of the tongue of forty-five male Wistar rats under anesthesia. Animals were randomly allocated to one of three groups based on the treatment: control, corticoid and copaiba. A daily dose of each treatment and vehicle was administrated by oral gavage for three consecutive days. Sample collections took place on the third, seventh and 15th days post-wounding for clinical and histopathological analyses. Blood was collected on the third and seventh days for kidneys and liver function tests. Semi-quantitative analyses were performed based on scores of inflammation and reepithelization. Tissue collagen deposition was detected by PicroSirius red staining. Copaiba-treated wounds revealed a smaller wound area, decreased of acute inflammatory reaction and enhanced reepithelization. The levels of kidney and liver function tests did not reveal presence of damage post-treatments. Our findings suggest that copaiba oleoresin is a safe and effective alternative therapy for inflammation and tissue repair of oral wounds in this animal model. |
