In Vitro and In Vivo Electrochemical Measurement of Reactive Oxygen Species After Treatment with Anticancer Drugs.

Autor: Vaneev AN; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia.; Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, 1, 3, Moscow 119991, Russia., Gorelkin PV; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia., Garanina AS; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia., Lopatukhina HV; Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, 1, 3, Moscow 119991, Russia., Vodopyanov SS; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia.; Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, 1, 3, Moscow 119991, Russia., Alova AV; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia.; Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, 1, 3, Moscow 119991, Russia., Ryabaya OO; N. N. Blokhin National Medical Research Center of Oncology, 24 Kashirskoe shosse, Moscow 115478, Russia., Akasov RA; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia.; I. M. Sechenov First Moscow State Medical University, Trubetskaya Str. 8-2, Moscow 119991, Russia., Zhang Y; Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.; Imperial College London, Department of Medicine, London W12 0NN, United Kingdom., Novak P; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia.; Imperial College London, Department of Medicine, London W12 0NN, United Kingdom., Salikhov SV; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia., Abakumov MA; N. I. Pirogov Russian National Research Medical University, Ostrovityanova Street 1/7, Moscow 117997, Russia., Takahashi Y; Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan., Edwards CRW; Imperial College London, Department of Medicine, London W12 0NN, United Kingdom., Klyachko NL; Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, 1, 3, Moscow 119991, Russia., Majouga AG; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia.; Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, 1, 3, Moscow 119991, Russia.; D. Mendeleev University of Chemical Technology of Russia, Miusskaya Square, 9, Moscow 125047, Russia., Korchev YE; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia.; Imperial College London, Department of Medicine, London W12 0NN, United Kingdom., Erofeev AS; National University of Science and Technology 'MISiS', Leninskiy Avenue, 4, Moscow 119049, Russia.; Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, 1, 3, Moscow 119991, Russia.
Jazyk: angličtina
Zdroj: Analytical chemistry [Anal Chem] 2020 Jun 16; Vol. 92 (12), pp. 8010-8014. Date of Electronic Publication: 2020 May 28.
DOI: 10.1021/acs.analchem.0c01256
Abstrakt: In vivo monitoring of reactive oxygen species (ROS) in tumors during treatment with anticancer therapy is important for understanding the mechanism of action and in the design of new anticancer drugs. In this work, a platinized nanoelectrode is placed into a single cell for detection of the ROS signal, and drug-induced ROS production is then recorded. The main advantages of this method are the short incubation time with the drug and its high sensitivity which allows the detection of low intracellular ROS concentrations. We have shown that our new method can measure the ROS response to chemotherapy in tumor-bearing mice in real-time. ROS levels were measured in vivo inside the tumor at different depths in response to doxorubicin. This work provides an effective new approach for the measurement of intracellular ROS by platinized nanoelectrodes.
Databáze: MEDLINE