Differential responses to kinase inhibition in FGFR2-addicted triple negative breast cancer cells: a quantitative phosphoproteomics study.

Autor: Cunningham DL; School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. d.cunningham@bham.ac.uk., Sarhan AR; School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.; Department of Medical Laboratory Techniques, Nasiriyah Technical Institute, Southern Technical University, Nasiriyah, 6400, Iraq., Creese AJ; School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.; Immunocore, 101 Park Drive, Milton Park, Abingdon, Oxfordshire, OX14 4RY, UK., Larkins KPB; School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK., Zhao H; School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK., Ferguson HR; School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.; Division of Molecular and Cellular Function, School of Biological Science, Faculty of Biology Medicine and Health, The University of Manchester, Manchester, M13 9PT, UK., Brookes K; School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK., Marusiak AA; School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.; Laboratory of Experimental Medicine, Centre of New Technologies, University of Warsaw, 02-097, Warszawa, Poland., Cooper HJ; School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK., Heath JK; School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. J.K.Heath@bham.ac.uk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 May 14; Vol. 10 (1), pp. 7950. Date of Electronic Publication: 2020 May 14.
DOI: 10.1038/s41598-020-64534-y
Abstrakt: Fibroblast Growth Factor (FGF) dependent signalling is frequently activated in cancer by a variety of different mechanisms. However, the downstream signal transduction pathways involved are poorly characterised. Here a quantitative differential phosphoproteomics approach, SILAC, is applied to identify FGF-regulated phosphorylation events in two triple- negative breast tumour cell lines, MFM223 and SUM52, that exhibit amplified expression of FGF receptor 2 (FGFR2) and are dependent on continued FGFR2 signalling for cell viability. Comparative Gene Ontology proteome analysis revealed that SUM52 cells were enriched in proteins associated with cell metabolism and MFM223 cells enriched in proteins associated with cell adhesion and migration. FGFR2 inhibition by SU5402 impacts a significant fraction of the observed phosphoproteome of these cells. This study expands the known landscape of FGF signalling and identifies many new targets for functional investigation. FGF signalling pathways are found to be flexible in architecture as both shared, and divergent, responses to inhibition of FGFR2 kinase activity in the canonical RAF/MAPK/ERK/RSK and PI3K/AKT/PDK/mTOR/S6K pathways are identified. Inhibition of phosphorylation-dependent negative-feedback pathways is observed, defining mechanisms of intrinsic resistance to FGFR2 inhibition. These findings have implications for the therapeutic application of FGFR inhibitors as they identify both common and divergent responses in cells harbouring the same genetic lesion and pathways of drug resistance.
Databáze: MEDLINE
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