Hylan G-F20 and galactomannan joint flares are associated to acute synovitis and release of inflammatory cytokines.

Autor: de Melo Nunes R; Departamento de Medicina Interna da Faculdade de Medicina da Universidade Federal do Ceará, Fortaleza, Ceará, Brazil., Cunha PLR; Departamento de Química Orgânica e Inorgânica da Universidade Federal do Ceará, Fortaleza, Brazil., Pinto ACMD; Departamento de Medicina Interna da Faculdade de Medicina da Universidade Federal do Ceará, Fortaleza, Ceará, Brazil., Girão VCC; Departamento de Morfologia da Faculdade de Medicina da Universidade Federal do Ceará, Fortaleza, Ceará, Brazil., de Andrade Feitosa JP; Departamento de Química Orgânica e Inorgânica da Universidade Federal do Ceará, Fortaleza, Brazil., Rocha FAC; Departamento de Medicina Interna da Faculdade de Medicina da Universidade Federal do Ceará, Fortaleza, Ceará, Brazil. arocha@ufc.br.
Jazyk: angličtina
Zdroj: Advances in rheumatology (London, England) [Adv Rheumatol] 2020 May 11; Vol. 60 (1), pp. 26. Date of Electronic Publication: 2020 May 11.
DOI: 10.1186/s42358-020-00127-7
Abstrakt: Background: Injection of Hylan G-F20 (HY) into joints may provoke local flares, which mechanisms may involve reaction to protein contaminants. We have previously developed a protein-free saline-soluble galactomannan derived from guar gum (GM) that displays both analgesia and chondroprotection in experimental osteoarthritis (OA). We now demonstrate that both GM and Hylan G-F20 (HY) promote mild synovitis with cytokine release after intra-articular injection.
Methods: Mice received 100 μg/25 μL GM or HY or saline into the knees. Joint pain was evaluated using von Frey test; cell influx, interleukin (IL)-1, IL-6, and CXCL-1 (pg/mL) levels were assessed in joint lavage at 6 h. Synovia were excised for histopathology.
Results: Neither GM nor HY after being given into mice knee joints induced pain albeit promoting mild cell influx into joint washings as well as mild synovitis at histology, with no damage to the underlying cartilage. HY but not GM promoted IL-1 release into mice joints. Both compounds induced IL-6 and CXCL-1 release.
Conclusion: Intra-articular injection of HY or GM promote acute transient synovitis whilst not provoking detectable significant joint damage. Local administration of these polysaccharides induces acute intra-articular release of inflammatory cytokines, which may account for joint flares following viscosupplementation.
Databáze: MEDLINE
Externí odkaz: