RT-qPCR study of COX-1 and -2 genes in oral surgical model comparing single-dose preemptive ibuprofen and etoricoxib: A randomized clinical trialy.

Autor:	Medeiros-Albuquerque AF; DDS, MSc, PhD, Division of Oral Surgery, School of Dentistry, Fortaleza University (UNIFOR), Fortaleza, Brazil., Roriz-Fonteles CS; DDS, MSc, PhD, Division of Clinical Dentistry, Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, Brazil., do Nascimento-Costa JJ; MSc, PhD, Biotechnology Nucleus of Sobral - NUBIS, School of Medicine, Federal University of Ceará, Sobral, Brazil., Viana-Silva JR; MSc, PhD, Biotechnology Nucleus of Sobral - NUBIS, School of Medicine, Federal University of Ceará, Sobral, Brazil., de Barros-Silva PG; DDS, MSc, PhD, Division of Clinical Dentistry, Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, Brazil., Studart-Soares EC; DDS, MSc, PhD, Division of Clinical Dentistry, Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, Brazil., Chaves FN; DDS, MSc, PhD, Division of Clinical Dentistry, Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, Brazil., Alves-Pereira KM; DDS, MSc, PhD, Division of Clinical Dentistry, Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, Brazil., Ribeiro TR; DDS, MSc, PhD, Division of Clinical Dentistry, Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, Brazil., Gurgel-Costa FW; DDS, MSc, PhD, Division of Clinical Dentistry, Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of clinical and experimental dentistry [J Clin Exp Dent] 2020 Apr 01; Vol. 12 (4), pp. e371-e380. Date of Electronic Publication: 2020 Apr 01 (Print Publication: 2020).
DOI:	10.4317/jced.56447
Abstrakt:	Background: This study aimed to evaluate the gene expression of cyclooxygenases (COXs) in an oral model of preemptive analgesia. Material and Methods: Gingival tissue was collected during extraction of lower third molars from a randomized, triple-blind, split-mouth and placebo-controlled study. The eligible patients were randomly sorted to receive a single dose either of ibuprofen 400mg, or etoricoxib 120 mg or a placebo, one hour prior to surgery. The temporal course of RNAm was evaluated for COX-1 and -2 by means of a quantitative polymerase chain reaction in real time (RT-qPCR) at time zero and 30 minutes after the surgical procedure began, and it was correlated with clinical parameters (pain and maximum mouth opening). Results: There was a significant increase in COX-1 expression between T0 and T30 in ibuprofen ( p =0.004) and etoricoxib ( p =0.010) groups. As regards COX-2, there were increases from T0 to T30 in all groups (placebo, p =0.012; ibuprofen, p <0.001; etoricoxib, p <0.001). All groups showed a significant decrease in COX-2:COX-1 ratio from T0 to T30 (placebo, p =0.013; ibuprofen, p <0.001; etoricoxib, p =0.047). Experimental groups showed a significant correlation between COX-1 and COX-2 levels and clinical pain parameters. Conclusions: The present preemptive analgesia study concludes that COX-2 RNAm induction was directly linked to third molar-related tissue inflammation and that the relation between COX-1 and COX-2 levels were inversely proportional to the preemptively administered nonsteroidal anti-inflammatory drugs COX-2 selectivity. Key words: Preemptive analgesia, dental extraction, cyclooxygenases, real-time polymerase chain reaction. Competing Interests: Conflicts of interest Non declared. (Copyright: © 2020 Medicina Oral S.L.)
Databáze:	MEDLINE
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