Histomorphometric and biochemical data of rat kidney submitted to warm ischemia associated with resveratrol treatment.

Autor:	Buys-Gonçalves GF; Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Sampaio FJB; Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Pereira-Sampaio MA; Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil.; Department of Morphology, Fluminense Federal University, Niteroi, Brazil., Silva MEM; Department of Veterinary Medicine, Educational Foundation Serra dos Órgãos, Teresópolis, Brazil., De Souza DB; Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Jazyk:	angličtina
Zdroj:	Data in brief [Data Brief] 2020 Apr 15; Vol. 30, pp. 105545. Date of Electronic Publication: 2020 Apr 15 (Print Publication: 2020).
DOI:	10.1016/j.dib.2020.105545
Abstrakt:	The data presented here come from the article "Histomorphometric evaluation of the rat kidney submitted to warm ischemia and the protective effect of resveratrol" [1]. Rats of Wistar lineage ( n  = 39; 9 weeks of age) were obtained and apportioned into 4 groups at random. Both groups Sham (S) and Sham Resveratrol (SR) were submitted to open laparotomy and dissection of the left renal pedicle, the same as groups Ischemia (I) and Ischemia Resveratrol (IR), being the last two also submitted to 1 h left warm renal ischemia. SR and IR were treated with 30 mg/kg of resveratrol intraperitoneally 1 h before the surgical procedure, while S and I received saline injections. Rats were killed a month after surgery by anesthetic overdose. A blood sample was collected by cardiac puncture for determination of serum urea and creatinine serum by biochemical analysis at automated enzymatic method. Kidneys were weighted, Sherle´s method was used for measurement of their volume and then both were fixated in buffered formalin for 48 h. Cortex-non-cortex areas ratio (C-NC) was assessed by Cavalieri's method using a stereoscope. The product of multiplying the renal volume by the C-NC is the cortical volume (CV). Left kidneys fragments were processed for histology resulting in slides that were stained with haematoxylin and eosin. For histomorphometric analyses, 25 random cortical fields were photographed at 200x magnification using a camera attached to a light microscope. The estimation of glomerular volumetric density (Vv [Glom]), indication of proportional volume occupied by glomeruli in the cortex, was performed by the point-counting method. The point-sampled intercepts method was used to estimate the volume-weighted mean glomerular volume (VWGV). Total number of glomeruli per kidney (N [Glom]) estimation was achieved through the formula CVxVv [Glom]/VWGV. All the data were tabulated in spreadsheets. The quantitative results were compared by one-way ANOVA with Tukey's post-test using GraphPad Prism software. All results were considered significant when the value of p <0.05. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships which have, or could be perceived to have, influenced the work reported in this article. (© 2020 The Authors.)
Databáze:	MEDLINE
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