Evaluation of xanthine oxidase inhibitory activity of flavonoids by an online capillary electrophoresis-based immobilized enzyme microreactor.
Autor: | Wu ZY; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China., Zhang H; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China., Li F; Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences, Chemistry, University of Tasmania, Hobart, Tasmania, Australia., Yang FQ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China. |
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Jazyk: | angličtina |
Zdroj: | Electrophoresis [Electrophoresis] 2020 Aug; Vol. 41 (15), pp. 1326-1332. Date of Electronic Publication: 2020 May 25. |
DOI: | 10.1002/elps.202000083 |
Abstrakt: | Xanthine oxidase (XOD) is a key enzyme in the human body to produce uric acid, and its inhibitor can be used for the treatment of hyperuricemia and gout. In this study, an online CE-based XOD immobilized enzyme microreactor (IMER) was developed for the enzyme kinetics assays and inhibitor screening. After 30 consecutive runs, the XOD activity remained about 95.6% of the initial immobilized activity. The Michaelis-Menten constant (K (© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
Databáze: | MEDLINE |
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