Evaluation of MALDI-TOF MS as a tool for detection of Listeria spp. directly from selective enrichment broth from food and stool samples.

Autor: Araújo TMC; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria, Rio de Janeiro/RJ, Brazil., Pereira RCL; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria, Rio de Janeiro/RJ, Brazil., Freitag IGR; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria, Rio de Janeiro/RJ, Brazil., Rusak LA; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Fisiologia Bacteriana, Rio de Janeiro/RJ, Brazil., Botelho LAB; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Prof. Paulo de Góes, Laboratório de Investigação em Microbiologia Médica, Rio de Janeiro/RJ, Brazil., Hofer E; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria, Rio de Janeiro/RJ, Brazil., Hofer CB; Universidade Federal do Rio de Janeiro, Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Rio de Janeiro/RJ, Brazil., Vallim DC; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria, Rio de Janeiro/RJ, Brazil. Electronic address: vallim@ioc.fiocruz.br.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2020 Jun; Vol. 173, pp. 105936. Date of Electronic Publication: 2020 Apr 30.
DOI: 10.1016/j.mimet.2020.105936
Abstrakt: We evaluated the detection of Listeria spp. using MALDI-TOF MS directly in enrichment broths, without isolated colonies, with naturally contaminated food and stool samples. The success rate was 77%. Considering the reduced time for diagnosis and the success rate, this is a promising screening tool, but more tests are needed to determine its viability.
Competing Interests: Declaration of Competing Interest The authors declare that we do not have any commercial interest in this work and have no conflict of interest with respect to the work represented in this article. This manuscript has not been simultaneously submitted for publication in another journal.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: