Weissella paramesenteroides WpK4 ameliorate the experimental amoebic colitis by increasing the expression of MUC-2 and the intestinal epithelial regeneration.

Autor: Prado GKS; Postgraduate Program in Pathology, Institute of Biological Sciences of the Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.; Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Torrinha KC; Postgraduate Program in Pathology, Institute of Biological Sciences of the Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Cruz RE; Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Gonçalves ABB; Postgraduate Program in Pathology, Institute of Biological Sciences of the Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.; Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Silva CAV; Postgraduate Program in Pathology, Institute of Biological Sciences of the Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.; Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Oliveira FMS; Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Nunes AC; Department of Genetics, Ecology and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Gomes MA; Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Caliari MV; Postgraduate Program in Pathology, Institute of Biological Sciences of the Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2020 Dec; Vol. 129 (6), pp. 1706-1719. Date of Electronic Publication: 2020 Jul 13.
DOI: 10.1111/jam.14671
Abstrakt: Aims: This study evaluates the action of Weissella paramesenteroides WpK4 on amoebic colitis.
Methods and Results: Weissella paramesenteroides WpK4 was administered in Entamoeba dispar infected and noninfected mice and clinical parameters were evaluated. Following 7 days, the caeca were collected for histopathology, morphometry and immunohistochemical staining of MUC-2, CDC-47 and IgA. The treatment reduced diarrhoea and the presence of blood in the faeces and diminished the area of necrosis, also causing weight gain. Also, the addition of this bacterium enhanced the expression of the mucin (MUC-2). The reduction in necrosis and increased CDC-47 expression indicates significant epithelial regeneration. The negative correlation between CDC-47 and the necrosis area reveals that the bacterium favoured the recovery of the necrotic regions and the positive correlation found between the expression of MUC-2 and CDC-47 indicates that the epithelial regeneration also supports the synthesis of MUC-2.
Conclusions: Weissella paramesenteroides WpK4 was able to increase the protection of the intestinal mucosa against experimental amoebic colitis through the increase of MUC-2 and epithelial regeneration.
Significance and Impact of the Study: Weissella paramesenteroides WpK4 presents the potential to become a complementary tool in the treatment of amoebic colitis.
(© 2020 The Society for Applied Microbiology.)
Databáze: MEDLINE
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