Aptasensors for viral diagnostics.

Autor: van den Kieboom CH; Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud University Medical Center, Nijmegen, Netherlands.; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands., van der Beek SL; Department of Medical Microbiology, University Medical Center Utrecht, Netherlands., Mészáros T; Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary.; MTA-BME Research Group for Technical Analytical Chemistry, Budapest University of Technology and Economics, Budapest, Hungary., Gyurcsányi RE; MTA-BME Lendület Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Budapest, Hungary., Ferwerda G; Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud University Medical Center, Nijmegen, Netherlands., de Jonge MI; Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud University Medical Center, Nijmegen, Netherlands.
Jazyk: angličtina
Zdroj: Trends in analytical chemistry : TRAC [Trends Analyt Chem] 2015 Dec; Vol. 74, pp. 58-67. Date of Electronic Publication: 2015 Oct 23.
DOI: 10.1016/j.trac.2015.05.012
Abstrakt: Novel viral diagnostic tools need to be affordable, fast, accurate and easy to use with sensitivity and specificity equivalent or superior to current standards. At present, viral diagnostics are based on direct detection of viral components or indirect detection by measuring antibodies generated in response to viral infection. While sensitivity of detection and quantification are still important challenges, we expect major advances from new assay formats and synthetic binding molecules, such as aptamers. Compared to traditional antibody-based detection, aptamers could provide faster adaptation to continuously evolving virus strains and higher discriminating capacity between specific virus serotypes. Aptamers are very stable and easily modifiable, so are ideal molecules for detection and chemical sensing applications. Here, we review the use of aptasensors for detection of viral pathogens and consider the feasibility of aptasensors to become standard devices for point-of-care diagnostics of viruses.
(Copyright © 2015 Published by Elsevier B.V.)
Databáze: MEDLINE
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