A perspective view on the nanomotion detection of living organisms and its features.

Autor: Venturelli L; Laboratoire de Physique de la Matière Vivante, Institut de Physique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Kohler AC; Laboratoire de Physique de la Matière Vivante, Institut de Physique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Stupar P; Laboratoire de Physique de la Matière Vivante, Institut de Physique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Villalba MI; Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI-CONICET-CCT La Plata), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina., Kalauzi A; Institute for Multidisciplinary Research, Department of Life Sciences, University of Belgrade, Belgrade, Serbia., Radotic K; Institute for Multidisciplinary Research, Department of Life Sciences, University of Belgrade, Belgrade, Serbia., Bertacchi M; Faculty of Medicine, University of Zurich, Zurich, Switzerland., Dinarelli S; Consiglio Nazionale delle Ricerche - Istituto di Struttura della Materia, CNR-ISM, Rome, Italy., Girasole M; Consiglio Nazionale delle Ricerche - Istituto di Struttura della Materia, CNR-ISM, Rome, Italy., Pešić M; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković' National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia., Banković J; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković' National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia., Vela ME; Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA-CONICET-CCT La Plata), Universidad Nacional de La Plata, La Plata, Argentina., Yantorno O; Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI-CONICET-CCT La Plata), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina., Willaert R; ARG VUB-UGent NanoMicrobiology, IJRG VUB-EPFL BioNanotechnology & NanoMedicine, Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.; Department of Bioscience Engineering, University of Antwerp, Antwerp, Belgium., Dietler G; Laboratoire de Physique de la Matière Vivante, Institut de Physique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Longo G; Consiglio Nazionale delle Ricerche - Istituto di Struttura della Materia, CNR-ISM, Rome, Italy., Kasas S; Laboratoire de Physique de la Matière Vivante, Institut de Physique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.; Centre Universitaire Romand de Médecine Légale, UFAM, Université de Lausanne, Lausanne, Switzerland.
Jazyk: angličtina
Zdroj: Journal of molecular recognition : JMR [J Mol Recognit] 2020 Dec; Vol. 33 (12), pp. e2849. Date of Electronic Publication: 2020 Mar 30.
DOI: 10.1002/jmr.2849
Abstrakt: The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.
(© 2020 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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