| Autor: |
Chakravarty S; Department of Radiology, Michigan State University, East Lansing, MI 48823, USA. shapir86@msu.edu., Hix JML, Wiewiora KA, Volk MC, Kenyon E, Shuboni-Mulligan DD, Blanco-Fernandez B, Kiupel M, Thomas J, Sempere LF, Shapiro EM |
| Jazyk: |
angličtina |
| Zdroj: |
Nanoscale [Nanoscale] 2020 Apr 14; Vol. 12 (14), pp. 7720-7734. Date of Electronic Publication: 2020 Mar 25. |
| DOI: |
10.1039/d0nr01234c |
| Abstrakt: |
Here, we describe the synthesis, characterization and in vitro and in vivo performance of a series of tantalum oxide (TaO x ) based nanoparticles (NPs) for computed tomography (CT). Five distinct versions of 9-12 nm diameter silane coated TaO x nanocrystals (NCs) were fabricated by a sol-gel method with varying degrees of hydrophilicity and with or without fluorescence, with the highest reported Ta content to date (78%). Highly hydrophilic NCs were left bare and were evaluated in vivo in mice for micro-CT of full body vasculature, where following intravenous injection, TaO x NCs demonstrate high vascular CT contrast, circulation in blood for ∼3 h, and eventual accumulation in RES organs; and following injection locally in the mammary gland, where the full ductal tree structure can be clearly delineated. Partially hydrophilic NCs were encapsulated within mesoporous silica nanoparticles (MSNPs; TaO x @MSNPs) and hydrophobic NCs were encapsulated within poly(lactic-co-glycolic acid) (PLGA; TaO x @PLGA) NPs, serving as potential CT-imagable drug delivery vehicles. Bolus intramuscular injections of TaO x @PLGA NPs and TaO x @MSNPs to mimic the accumulation of NPs at a tumor site produce high signal enhancement in mice. In vitro studies on bare NCs and formulated NPs demonstrate high cytocompatibility and low dissolution of TaO x . This work solidifies that TaO x -based NPs are versatile contrast agents for CT. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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