Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphate.

Autor:	Dersjant-Li Y; DuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the Netherlands., Villca B; Institut de Recerca I Tecnologia Agroalimentàries, Centre Mas de Bover, Ctra. Reus-El Morell km. 3.8, E-43120, Constantí, Spain., Sewalt V; DuPont Nutrition & Biosciences, 925 Page Mill Road, Palo Alto, CA 94304, USA., de Kreij A; DuPont Nutrition & Biosciences, 21 Biopolis Road, Nucleos, South Tower, 138567, Singapore., Marchal L; DuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the Netherlands., Velayudhan DE; DuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the Netherlands., Sorg RA; DuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the Netherlands., Christensen T; DuPont Nutrition and Biosciences, Edwin Rahrs Vej 38, DK-8220, Brabrand, Denmark., Mejldal R; DuPont Nutrition and Biosciences, Edwin Rahrs Vej 38, DK-8220, Brabrand, Denmark., Nikolaev I; DuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the Netherlands., Pricelius S; DuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the Netherlands., Kim HS; DuPont Nutrition & Biosciences, 925 Page Mill Road, Palo Alto, CA 94304, USA., Haaning S; DuPont Nutrition and Biosciences, Edwin Rahrs Vej 38, DK-8220, Brabrand, Denmark., Sørensen JF; DuPont Nutrition and Biosciences, Edwin Rahrs Vej 38, DK-8220, Brabrand, Denmark., Lizardo R; Institut de Recerca I Tecnologia Agroalimentàries, Centre Mas de Bover, Ctra. Reus-El Morell km. 3.8, E-43120, Constantí, Spain.
Jazyk:	angličtina
Zdroj:	Animal nutrition (Zhongguo xu mu shou yi xue hui) [Anim Nutr] 2020 Mar; Vol. 6 (1), pp. 24-30. Date of Electronic Publication: 2019 Dec 10.
DOI:	10.1016/j.aninu.2019.11.003
Abstrakt:	The utility of a next generation biosynthetic bacterial 6-phytase (PhyG) in restoring bone ash, bone phosphorus (P) content and performance in piglets depleted in P was evaluated. A total of 9 treatments were tested as follows. Treatment 1, a negative control (NC) diet; treatments 2, 3, 4, NC supplemented with 250, 500 or 1,000 FTU/kg of PhyG; treatments 5, 6, NC supplemented with 500 or 1,000 FTU/kg of a commercial Buttiauxella sp phytase (PhyB); treatments 7, 8, 9, NC supplemented with monocalcium phosphate (MCP) to provide 0.7, 1.4 and 1.8 g/kg digestible P, equating to a digestible P content of 1.8, 2.5 and 2.9 g/kg. The latter constituting the positive control (PC) diet with adequate P and calcium (Ca). The NC was formulated without inorganic P (1.1 g digestible P/kg) and reduced in Ca (5.0 g/kg). Additional limestone was added to treatments 7 to 9 to maintain Ca-to-P ratio between 1.2 and 1.3. A total of 162 crossed Pietrain × (Large White × Landrace) 21-d-old piglets (50% males and 50% females) were fed adaptation diets until 42 d old and then assigned to pens with 2 pigs/pen and 9 pens/treatment in a completely randomized block design. Piglets were fed mash diets based on corn and soybean meal ad libitum for 28 d. At the end of the study, one piglet perpen was euthanized and the right feet collected for determination of bone strength, bone ash and mineral content. Compared with the PC, the NC group had reduced average daily gain (ADG) and increased feed conversion ratio (FCR) during all growth phases and overall, and at d 28 (70 d old) NC pigs had bones with reduced ash, Ca and P content ( P  < 0.05). The PhyG at 250 FTU/kg improved bone ash vs. NC. Increasing PhyG dose linearly or quadratically improved bone ash, ADG and FCR ( P  < 0.05). At ≥ 500 FTU/kg, both PhyG and PhyB maintained ADG and FCR equivalent to PC. Linear regression analysis was done to compare the measured response parameters to increasing digestible P from MCP. Based on this analysis it was shown that PhyG and PhyB at 1,000 FTU/kg could replace 1.83 and 1.66 g/kg digestible P from MCP in the diet, respectively, on average across metacarpi bone ash, ADG or FCR. These findings suggest that the biosynthetic phytase is highly effective in the tested dietary setting. Competing Interests: Yueming Dersjant-Li, Vincent Sewalt, Arno de Kreij, Leon Marchal, Deepak, E. Velayudhan, Robin Anton Sorg, Trine Christensen, Rie Mejldal, Igor Nikolaev, Sina Pricelius, Hye-Sook Kim, Svend Haaning, Jens Frisbæk Sørensen are employee of DuPont Nutrition & Biosciences. We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the content of this paper. (© 2020 Chinese Association of Animal Science and Veterinary Medicine. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.)
Databáze:	MEDLINE
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