The Maize Hairy Sheath Frayed1 ( Hsf1 ) Mutation Alters Leaf Patterning through Increased Cytokinin Signaling.

Autor:	Muszynski MG; Department of Tropical Plant and Soil Sciences, University of Hawai'i at Mānoa, Honolulu, Hawaii 96822 mgmuszyn@hawaii.edu., Moss-Taylor L; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011., Chudalayandi S; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011., Cahill J; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011., Del Valle-Echevarria AR; Department of Tropical Plant and Soil Sciences, University of Hawai'i at Mānoa, Honolulu, Hawaii 96822., Alvarez-Castro I; Department of Statistics, Iowa State University, Ames, Iowa 50011., Petefish A; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011., Sakakibara H; RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama 230-0045, Japan., Krivosheev DM; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia., Lomin SN; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia., Romanov GA; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia., Thamotharan S; School of Chemical and Biotechnology, SASTRA University; Thanjavur, 613401, India., Dam T; Corteva Agriscience, Johnston, Iowa 50131., Li B; Corteva Agriscience, Johnston, Iowa 50131., Brugière N; Corteva Agriscience, Johnston, Iowa 50131.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2020 May; Vol. 32 (5), pp. 1501-1518. Date of Electronic Publication: 2020 Mar 23.
DOI:	10.1105/tpc.19.00677
Abstrakt:	Leaf morphogenesis requires growth polarized along three axes-proximal-distal (P-D) axis, medial-lateral axis, and abaxial-adaxial axis. Grass leaves display a prominent P-D polarity consisting of a proximal sheath separated from the distal blade by the auricle and ligule. Although proper specification of the four segments is essential for normal morphology, our knowledge is incomplete regarding the mechanisms that influence P-D specification in monocots such as maize ( Zea mays ). Here, we report the identification of the gene underlying the semidominant, leaf patterning maize mutant Hairy Sheath Frayed1 ( Hsf1 ). Hsf1 plants produce leaves with outgrowths consisting of proximal segments-sheath, auricle, and ligule-emanating from the distal blade margin. Analysis of three independent Hsf1 alleles revealed gain-of-function missense mutations in the ligand binding domain of the maize cytokinin (CK) receptor Z. mays Histidine Kinase1 ( ZmHK1 ) gene. Biochemical analysis and structural modeling suggest the mutated residues near the CK binding pocket affect CK binding affinity. Treatment of the wild-type seedlings with exogenous CK phenocopied the Hsf1 leaf phenotypes. Results from expression and epistatic analyses indicated the Hsf1 mutant receptor appears to be hypersignaling. Our results demonstrate that hypersignaling of CK in incipient leaf primordia can reprogram developmental patterns in maize. (© 2020 American Society of Plant Biologists. All rights reserved.)
Databáze:	MEDLINE
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