| Autor: |
Bowen BJ; School of Biosciences, Cardiff University, Cardiff, UK. jonesdd@cardiff.ac.uk., McGarrity AR, Szeto JA, Pudney CR, Jones DD |
| Jazyk: |
angličtina |
| Zdroj: |
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2020 Apr 21; Vol. 56 (31), pp. 4308-4311. Date of Electronic Publication: 2020 Mar 18. |
| DOI: |
10.1039/d0cc00596g |
| Abstrakt: |
Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Using computational design, we converted metalloporphyrin specificity of cytochrome b 562 from iron to fluorogenic zinc. The new variant had a near total preference for zinc representing a switch in specificity, which greatly enhanced the negligible aqueous fluorescence of free ZnPP in vitro and in vivo. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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