[Cytokine-regulating activity of anti-virus preparation CelAgripus in Burkitt's lymphoma stable B-cell lines.]
| Autor: | Narovlyansky AN; National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Moscow, 123098, Russian Federation., Mezentseva MV; National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Moscow, 123098, Russian Federation., Suetina IA; National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Moscow, 123098, Russian Federation., Russu LI; National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Moscow, 123098, Russian Federation., Ivanova AM; National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Moscow, 123098, Russian Federation., Poloskov VV; National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Moscow, 123098, Russian Federation., Izmest'eva AV; National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Moscow, 123098, Russian Federation., Ospelnikova TP; National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Moscow, 123098, Russian Federation., Sarymsakov AA; Institute of Polymer Chemystry and Physics Uzbek Academy of Sciences, Tashkent, 100128, Uzbekistan., Ershov FI; National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Moscow, 123098, Russian Federation. |
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| Jazyk: | ruština |
| Zdroj: | Voprosy virusologii [Vopr Virusol] 2019; Vol. 64 (4), pp. 165-172. |
| DOI: | 10.36233/0507-4088-2019-64-4-165-172 |
| Abstrakt: | Introduction: Cytokines activated in response to immunosuppressive viral infections can directly or indirectly affect the neoplastic transformation of B cells. In this study, we studied a new substance designed to produce the antiviral drug CelAgrip (CA, CelAgripus), which exhibits interferon (IFN) and cytokine-inducing activity and, apparently, can be used as an activator of antiviral immunity. Purpose - is to evaluate the cytokine-regulating effect of CA in Burkitt's lymphoma (LB) cell lines latently infected with the Epstein-Barr virus (EBV). Objectives: to study the CA-induced expression of the cytokine genes IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, IL-18, IFN-α, IFN -γ, IFN-β, IFN-λ1, IFN-λ2, IFN-λ3, TNF-α in normal and EBV transformed LB cells. Material and Methods: Cell line: the human embryo fibroblasts (HEF), Namalva, Daudi, Raji, P3HR-1. Preparations: CA, gossypol-acetic acid (GAA), sodium carboxymethyl cellulose (Na-CMC). Methods: RT-PCR and methods for assessing cytotoxicity (MTT and Scepter 2.0 Merck cell counter). Results: The effect of the CA preparation on the expression of IFN-λ, IL-1β, IL-6, IL-8 and IL-10 genes was revealed. Discussion: We observed the activation of gene expression of IFN-λ, IL-1β, IL-6, IL-8 and suppression of IL-10 gene activity when treatment CA of LB cells. Conclusion: The substance CA has new effects on the activation of the expression of a number of key cytokine genes in stable Burkitt lymphoma cell lines. Competing Interests: The authors declare no conflict of interest. |
| Databáze: | MEDLINE |
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