The temporal structure of the inner retina at a single glance.

Autor: Zhao Z; Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.; Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany., Klindt DA; Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.; Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany.; Bernstein Centre for Computational Neuroscience, University of Tübingen, Tübingen, Germany.; Graduate Training Centre of Neuroscience, University of Tübingen, Tübingen, Germany.; Institute for Theoretical Physics, University of Tübingen, Tübingen, Germany., Maia Chagas A; Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.; Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany.; Graduate Training Centre of Neuroscience, University of Tübingen, Tübingen, Germany., Szatko KP; Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.; Bernstein Centre for Computational Neuroscience, University of Tübingen, Tübingen, Germany.; Graduate Training Centre of Neuroscience, University of Tübingen, Tübingen, Germany., Rogerson L; Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.; Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany.; Bernstein Centre for Computational Neuroscience, University of Tübingen, Tübingen, Germany.; Graduate Training Centre of Neuroscience, University of Tübingen, Tübingen, Germany., Protti DA; Department of Physiology and Bosch Institute, The University of Sydney, Sydney, Australia., Behrens C; Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.; Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany.; Bernstein Centre for Computational Neuroscience, University of Tübingen, Tübingen, Germany., Dalkara D; Sorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France., Schubert T; Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.; Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany., Bethge M; Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany.; Bernstein Centre for Computational Neuroscience, University of Tübingen, Tübingen, Germany.; Institute for Theoretical Physics, University of Tübingen, Tübingen, Germany.; Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA., Franke K; Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.; Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany.; Bernstein Centre for Computational Neuroscience, University of Tübingen, Tübingen, Germany., Berens P; Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.; Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany.; Bernstein Centre for Computational Neuroscience, University of Tübingen, Tübingen, Germany.; Institute of Bioinformatics and Medical Informatics, University of Tübingen, Tübingen, Germany., Ecker AS; Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany.; Bernstein Centre for Computational Neuroscience, University of Tübingen, Tübingen, Germany.; Institute for Theoretical Physics, University of Tübingen, Tübingen, Germany.; Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA.; Department of Computer Science, University of Göttingen, Göttingen, Germany., Euler T; Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany. thomas.euler@cin.uni-tuebingen.de.; Bernstein Centre for Computational Neuroscience, University of Tübingen, Tübingen, Germany. thomas.euler@cin.uni-tuebingen.de.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Mar 10; Vol. 10 (1), pp. 4399. Date of Electronic Publication: 2020 Mar 10.
DOI: 10.1038/s41598-020-60214-z
Abstrakt: The retina decomposes visual stimuli into parallel channels that encode different features of the visual environment. Central to this computation is the synaptic processing in a dense layer of neuropil, the so-called inner plexiform layer (IPL). Here, different types of bipolar cells stratifying at distinct depths relay the excitatory feedforward drive from photoreceptors to amacrine and ganglion cells. Current experimental techniques for studying processing in the IPL do not allow imaging the entire IPL simultaneously in the intact tissue. Here, we extend a two-photon microscope with an electrically tunable lens allowing us to obtain optical vertical slices of the IPL, which provide a complete picture of the response diversity of bipolar cells at a "single glance". The nature of these axial recordings additionally allowed us to isolate and investigate batch effects, i.e. inter-experimental variations resulting in systematic differences in response speed. As a proof of principle, we developed a simple model that disentangles biological from experimental causes of variability and allowed us to recover the characteristic gradient of response speeds across the IPL with higher precision than before. Our new framework will make it possible to study the computations performed in the central synaptic layer of the retina more efficiently.
Databáze: MEDLINE