A novel bivalent Pasteurellosis-RHD vaccine candidate adjuvanted with Montanide ISA70 protects rabbits from lethal challenge.

Autor:	El-Jakee JK; Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt., Moussa IM; Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.; Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt., Omran MS; Department of Autogenous Vaccine, EGYVET, VACCSERA, Dokki 12611, Giza, Egypt., Ahmed BM; Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt., Elgamal MA; Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt., Hemeg HA; Department of Medical Technology/Microbiology, College of Applied Medical Science, Taibah University, Madinah, Saudi Arabia., Mubarak AS; Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia., Al-Maary KS; Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia., Kabli SA; Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia., Marouf SA; Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt., Haji Alhaaji J; Department of Health Science, College of Applied Studies and Community Service, King Saud University, Saudi Arabia.
Jazyk:	angličtina
Zdroj:	Saudi journal of biological sciences [Saudi J Biol Sci] 2020 Mar; Vol. 27 (3), pp. 996-1001. Date of Electronic Publication: 2020 Jan 08.
DOI:	10.1016/j.sjbs.2019.12.042
Abstrakt:	In the present study, a bivalent vaccine against Pasteurella multocida and rabbit hemorrhagic disease virus (RHDV) was formulated with Montanide™ ISA70 oil adjuvant (Seppic, Paris, France). Its efficacy was evaluated and compared to similar monovalent preparations and commercially available monovalent vaccines. White new Zeeland rabbit groups (n = 10) received 2 successive doses of the tested vaccines and were challenged 2 weeks after 2nd dose with Pasteurella multocida and RHDV or either pathogens according to their vaccination schedule. Challenged not-vaccinated group of rabbits (n = 10) was included as a control. The bivalent and monovalent ISA70 preparations were found stable, safe, sterile, pure and of low viscosity. Group 3 (GP3) which received bivalent vaccine showed the highest antibody geometric mean titers against Pasteurella multocida and RHDV evaluated by ELISA and hemagglutination inhibition (HI) respectively. Following virulent challenge; Gp3 rabbits were 90% protected from challenge over other groups that showed 80% protection. Detection of either pathogen in the livers of dead and euthanized rabbits had failed except for non-vaccinated controls. The bivalent vaccine candidate was fully protective. Immunization against both pathogens can be achieved by single vaccination. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2020 The Author(s).)
Databáze:	MEDLINE
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