Chromatographic separation simulation of metal-chelating peptides from surface plasmon resonance binding parameters.

Autor: Muhr L; Université de Lorraine, Ecole Nationale Supérieure des Industries Chimiques, Laboratoire Réactions et Génie des Procédés (UMR CNRS-UL 7274), 1 rue Grandville, Nancy, 54000, France., Pontvianne S; Université de Lorraine, Ecole Nationale Supérieure des Industries Chimiques, Laboratoire Réactions et Génie des Procédés (UMR CNRS-UL 7274), 1 rue Grandville, Nancy, 54000, France., Selmeczi K; Université de Lorraine, Laboratoire Lorrain de Chimie Moléculaire UMR 7053 CNRS-UL, Boulevard des Aiguillettes, 54506 Vandoeuvre-lès-Nancy cedex, Nancy cedex, BP, 70239, France., Paris C; Université de Lorraine, PASM platform, ENSAIA French engineering school, 2, avenue de la Forêt de Haye, Vandoeuvre-lès-Nancy, 54000, France., Boschi-Muller S; Université de Lorraine, Laboratoire IMoPA, Vandoeuvre-lès-Nancy cedex, 54506, France., Canabady-Rochelle L; Université de Lorraine, Ecole Nationale Supérieure des Industries Chimiques, Laboratoire Réactions et Génie des Procédés (UMR CNRS-UL 7274), 1 rue Grandville, Nancy, 54000, France.
Jazyk: angličtina
Zdroj: Journal of separation science [J Sep Sci] 2020 Jun; Vol. 43 (11), pp. 2031-2041. Date of Electronic Publication: 2020 Apr 02.
DOI: 10.1002/jssc.201900882
Abstrakt: Some metal-chelating peptides have antioxidant properties, with potential nutrition, health, and cosmetics applications. This study aimed to simulate their separation on immobilized metal ion affinity chromatography from their affinity constant for immobilized metal ion determined in surface plasmon resonance, both technics are based on peptide-metal ion interactions. In our approach, first, the affinity constant of synthetic peptides was determined by surface plasmon resonance and used as input data to numerically simulate the chromatographic separation with a transport-dispersive model based on Langmuir adsorption isotherm. Then, chromatographic separation was applied on the same peptides to determine their retention time and compare this experimental t R with the simulated t R obtained from simulation from surface plasmon resonance data. For the investigated peptides, the relative values of t R were comparable. Hence, our study demonstrated the pertinence of such numerical simulation correlating immobilized metal ion affinity chromatography and surface plasmon resonance.
(© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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