| Autor: |
M Weerakoon-Ratnayake K; Department of Chemistry, The University of Kansas, Lawrence, KS 66047, USA.; Center of BioModular Multiscale Systems for Precision Medicine, Lawrence, KS 66045, USA., Vaidyanathan S; Center of BioModular Multiscale Systems for Precision Medicine, Lawrence, KS 66045, USA.; Bioengineering, The University of Kansas, Lawrence, KS 66045, USA., Larky N; Center of BioModular Multiscale Systems for Precision Medicine, Lawrence, KS 66045, USA.; Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA., Dathathreya K; Department of Chemistry, The University of Kansas, Lawrence, KS 66047, USA.; Center of BioModular Multiscale Systems for Precision Medicine, Lawrence, KS 66045, USA., Hu M; Center of BioModular Multiscale Systems for Precision Medicine, Lawrence, KS 66045, USA.; Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA., Jose J; Center of BioModular Multiscale Systems for Precision Medicine, Lawrence, KS 66045, USA., Mog S; Department of Chemistry, The University of Kansas, Lawrence, KS 66047, USA.; Center of BioModular Multiscale Systems for Precision Medicine, Lawrence, KS 66045, USA., August K; Children's Mercy Hospital, Kansas City, MO 64108, USA., K Godwin A; Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA., L Hupert M; Biofluidica Inc., BioFluidica Research Laboratory, Lawrence, KS 66047, USA., A Witek M; Department of Chemistry, The University of Kansas, Lawrence, KS 66047, USA.; Center of BioModular Multiscale Systems for Precision Medicine, Lawrence, KS 66045, USA., A Soper S; Department of Chemistry, The University of Kansas, Lawrence, KS 66047, USA.; Center of BioModular Multiscale Systems for Precision Medicine, Lawrence, KS 66045, USA.; Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA.; Biofluidica Inc., BioFluidica Research Laboratory, Lawrence, KS 66047, USA.; Department of Mechanical Engineering, The University of Kansas, Lawrence, KS 66045, USA. |
| Abstrakt: |
The role of circulating plasma cells (CPCs) and circulating leukemic cells (CLCs) as biomarkers for several blood cancers, such as multiple myeloma and leukemia, respectively, have recently been reported. These markers can be attractive due to the minimally invasive nature of their acquisition through a blood draw (i.e., liquid biopsy), negating the need for painful bone marrow biopsies. CPCs or CLCs can be used for cellular/molecular analyses as well, such as immunophenotyping or fluorescence in situ hybridization (FISH). FISH, which is typically carried out on slides involving complex workflows, becomes problematic when operating on CLCs or CPCs due to their relatively modest numbers. Here, we present a microfluidic device for characterizing CPCs and CLCs using immunofluorescence or FISH that have been enriched from peripheral blood using a different microfluidic device. The microfluidic possessed an array of cross-channels (2-4 µm in depth and width) that interconnected a series of input and output fluidic channels. Placing a cover plate over the device formed microtraps, the size of which was defined by the width and depth of the cross-channels. This microfluidic chip allowed for automation of immunofluorescence and FISH, requiring the use of small volumes of reagents, such as antibodies and probes, as compared to slide-based immunophenotyping and FISH. In addition, the device could secure FISH results in <4 h compared to 2-3 days for conventional FISH. |
