Multiple crystallization as a potential strategy for efficient recovery of succinic acid following fermentation with immobilized cells.

Autor: Luthfi AAI; Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia., Tan JP; Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia., Isa NFAM; Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia., Bukhari NA; Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia.; Energy and Environment Unit, Engineering & Processing Research Division, Malaysian Palm Oil Board (MPOB), 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia., Shah SSM; Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia., Mahmod SS; Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia., Jahim JM; Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia. jamal@ukm.edu.my.; Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia. jamal@ukm.edu.my.
Jazyk: angličtina
Zdroj: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2020 Jul; Vol. 43 (7), pp. 1153-1169. Date of Electronic Publication: 2020 Feb 24.
DOI: 10.1007/s00449-020-02311-x
Abstrakt: This study aimed to enhance the crystallizability of bio-based succinic acid for its efficient recovery while maintaining the end product at the highest purity. Immobilization of Actinobacillus succinogenes was initially evaluated based on three different carriers: volcanic glass, clay pebbles, and silica particles. The adsorption capacity of metabolites with a low concentration (10 g/L) and a high concentration (40 g/L) was investigated. It was demonstrated that clay pebbles adsorbed the least succinic acid (< 11 mg/g clay pebbles). The repeated batch-fermentation trials with immobilized cells highlighted that succinic acid with an average concentration of up to 36.3 g/L with a metabolite-production ratio of 3:1 (succinic acid to by-products) could be attained within 130 h. Subsequently, the purification of succinic acid through crystallization was assessed in terms of pH, temperature, crystallization time, initial succinic acid concentration and multiple recrystallization processes. Increasing the crystallization time from 6 h to 9 h afforded an improvement of 17% in the recovery of succinic acid crystals. Moreover, a fourfold concentration coefficient of the broth yielded the highest purity percentage (99.9%). The crystallization in three consecutive stages at 9 h (with a fourfold concentration coefficient) successfully improved the total recovery percentage of succinic acid from 55.0 to 84.8%.
Databáze: MEDLINE
Externí odkaz: