Primary and secondary anti-viral response captured by the dynamics and phenotype of individual T cell clones.

Autor:	Minervina AA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation., Pogorelyy MV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation.; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow, Russian Federation., Komech EA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation.; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow, Russian Federation., Karnaukhov VK; Center of Life Sciences, Skoltech, Moscow, Russian Federation., Bacher P; Institute of Immunology, Kiel University, Kiel, Germany., Rosati E; Institute of Clinical Molecular Biology, Kiel University, Kiel, Germany., Franke A; Institute of Clinical Molecular Biology, Kiel University, Kiel, Germany., Chudakov DM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation.; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow, Russian Federation.; Center of Life Sciences, Skoltech, Moscow, Russian Federation.; Masaryk University, Central European Institute of Technology, Brno, Czech Republic., Mamedov IZ; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation.; Masaryk University, Central European Institute of Technology, Brno, Czech Republic.; V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russian Federation., Lebedev YB; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation.; Moscow State University, Moscow, Russian Federation., Mora T; Laboratoire de physique de l'École normale supérieure, ENS, PSL, Sorbonne Université, Université de Paris, and CNRS, Paris, France., Walczak AM; Laboratoire de physique de l'École normale supérieure, ENS, PSL, Sorbonne Université, Université de Paris, and CNRS, Paris, France.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2020 Feb 21; Vol. 9. Date of Electronic Publication: 2020 Feb 21.
DOI:	10.7554/eLife.53704
Abstrakt:	The diverse repertoire of T-cell receptors (TCR) plays a key role in the adaptive immune response to infections. Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization - the model for acute infection in humans - showing their large diversity. We confirm the secondary response is an order of magnitude weaker, albeit ∼10 days faster than the primary one. Estimating the fraction of the T-cell response directed against the single immunodominant epitope, we identify the sequence features of TCRs that define the high precursor frequency of the two major TCR motifs specific for this particular epitope. We also show the consistency of clonal expansion dynamics between bulk alpha and beta repertoires, using a new methodology to reconstruct alpha-beta pairings from clonal trajectories. Competing Interests: AM, MP, EK, VK, PB, ER, AF, DC, IM, YL, TM No competing interests declared, AW Senior editor, eLife (© 2020, Minervina et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu