The Immune Microenvironment and Neoantigen Landscape of Aggressive Salivary Gland Carcinomas Differ by Subtype.

Autor:	Linxweiler M; Human Oncology and Pathology Program, Memorial Sloan Kettering Cancer Center, New York, New York., Kuo F; Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, New York., Katabi N; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York., Lee M; Human Oncology and Pathology Program, Memorial Sloan Kettering Cancer Center, New York, New York., Nadeem Z; Human Oncology and Pathology Program, Memorial Sloan Kettering Cancer Center, New York, New York., Dalin MG; Human Oncology and Pathology Program, Memorial Sloan Kettering Cancer Center, New York, New York.; Department of Pediatrics, Institute of Clinical Sciences, University of Gothenburg, Gothenburg, Sweden., Makarov V; Human Oncology and Pathology Program, Memorial Sloan Kettering Cancer Center, New York, New York.; Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, New York., Chowell D; Human Oncology and Pathology Program, Memorial Sloan Kettering Cancer Center, New York, New York.; Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, New York., Dogan S; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York., Ganly I; Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York., Hakimi AA; Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York., Wong RJ; Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York., Riaz N; Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, New York.; Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center, New York, New York., Ho AL; Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York., Chan TA; Human Oncology and Pathology Program, Memorial Sloan Kettering Cancer Center, New York, New York. morrisl@mskcc.org chant@mskcc.org.; Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, New York.; Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center, New York, New York., Morris LGT; Human Oncology and Pathology Program, Memorial Sloan Kettering Cancer Center, New York, New York. morrisl@mskcc.org chant@mskcc.org.; Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, New York.; Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York.
Jazyk:	angličtina
Zdroj:	Clinical cancer research : an official journal of the American Association for Cancer Research [Clin Cancer Res] 2020 Jun 15; Vol. 26 (12), pp. 2859-2870. Date of Electronic Publication: 2020 Feb 14.
DOI:	10.1158/1078-0432.CCR-19-3758
Abstrakt:	Purpose: Salivary gland carcinomas (SGC) are rare, aggressive cancers with high rates of recurrence and distant metastasis. These factors, and a lack of active systemic therapies, contribute to poor clinical outcome. Response rates with immune checkpoint blockade have been low, although clinical data remain sparse. To improve the efficacy of therapies, a more comprehensive understanding of relevant molecular alterations and immunologic processes is needed. Experimental Design: To characterize the immune microenvironment and neoantigen landscape of SGCs, we performed RNA sequencing (RNA-seq) in 76 tumors representing the three most lethal histologies: adenoid cystic carcinoma (ACC), myoepithelial carcinoma (MECA), and salivary duct carcinoma (SDC). We analyzed transcriptomic profiles, tumor-infiltrating immune cell populations, and measures of T-cell activation/dysfunction. In 37 cases also undergoing exome sequencing, we analyzed somatic mutations and neoantigens. Results: SDCs exhibited high levels of immune infiltration, with corresponding higher levels of T-cell dysfunction, and higher mutational load. In contrast, ACCs were characterized by an immune-excluded microenvironment, the presence of M2-polarized macrophages and myeloid-derived suppressor cells, and very low mutational load. MECAs were more heterogeneous, with both immune-low and immune-high phenotypes represented. Across all SGCs, levels of immune infiltration were associated with mutation- and fusion-derived neoantigens, and with aggressive clinical behavior. Conclusions: These findings provide new insights into the immune microenvironment and neoantigen landscape of SGCs, showing that mechanisms of immune escape appear to differ by histology. These data nominate potential immunologic vulnerabilities and may help guide the next steps of investigation in precision immunotherapy for these difficult-to-treat cancers. (©2020 American Association for Cancer Research.)
Databáze:	MEDLINE
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