Polysaccharide Structures in the Outer Mucilage of Arabidopsis Seeds Visualized by AFM.

Autor: Williams MAK; School of Fundamental Sciences, Massey University, PN461, Private Bag 11222, Palmerston North 4442, New Zealand.; MacDiarmid Institute for Advanced Materials and Nanotechnology, Victoria University of Wellington, P.O. Box 600, Wellington 6140, New Zealand.; The Riddet Institute, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand., Cornuault V; School of Fundamental Sciences, Massey University, PN461, Private Bag 11222, Palmerston North 4442, New Zealand.; The Riddet Institute, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand., Irani AH; School of Fundamental Sciences, Massey University, PN461, Private Bag 11222, Palmerston North 4442, New Zealand.; MacDiarmid Institute for Advanced Materials and Nanotechnology, Victoria University of Wellington, P.O. Box 600, Wellington 6140, New Zealand., Symonds VV; School of Fundamental Sciences, Massey University, PN461, Private Bag 11222, Palmerston North 4442, New Zealand., Malmström J; MacDiarmid Institute for Advanced Materials and Nanotechnology, Victoria University of Wellington, P.O. Box 600, Wellington 6140, New Zealand.; Department of Chemical and Materials Engineering, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand., An Y; Department of Chemical and Materials Engineering, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand., Sims IM; The Ferrier Research Institute, Victoria University of Wellington, P.O. Box 33-436, Petone 5046, New Zealand., Carnachan SM; The Ferrier Research Institute, Victoria University of Wellington, P.O. Box 33-436, Petone 5046, New Zealand., Sallé C; Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000, Versailles, France., North HM; Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000, Versailles, France.
Jazyk: angličtina
Zdroj: Biomacromolecules [Biomacromolecules] 2020 Apr 13; Vol. 21 (4), pp. 1450-1459. Date of Electronic Publication: 2020 Mar 04.
DOI: 10.1021/acs.biomac.9b01756
Abstrakt: Evidence is presented that the polysaccharide rhamnogalacturonan I (RGI) can be biosynthesized in remarkably organized branched configurations and surprisingly long versions and can self-assemble into a plethora of structures. AFM imaging has been applied to study the outer mucilage obtained from wild-type (WT) and mutant ( bxl1-3 and cesa5-1 ) Arabidopsis thaliana seeds. For WT mucilage, ordered, multichain structures of the polysaccharide RGI were observed, with a helical twist visible in favorable circumstances. Molecular dynamics (MD) simulations demonstrated the stability of several possible multichain complexes and the possibility of twisted fibril formation. For bxl1-3 seeds, the imaged polymers clearly showed the presence of side chains. These were surprisingly regular and well organized with an average length of ∼100 nm and a spacing of ∼50 nm. The heights of the side chains imaged were suggestive of single polysaccharide chains, while the backbone was on average 4 times this height and showed regular height variations along its length consistent with models of multichain fibrils examined in MD. Finally, in mucilage extracts from cesa5-1 seeds, a minor population of chains in excess of 30 μm long was observed.
Databáze: MEDLINE
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