Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10.

Autor:	Pacheco FS; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Prata RBDS; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Brandão SS; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Ferreira H; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Rodrigues TF; Cellular Microbiology Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil; and., Brandão Dos Santos J; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., da Silva CO; Cellular Microbiology Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil; and., Tavares IF; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Mendes MA; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Rodrigues ACDP; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Machado AM; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Nery JADC; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Amadeu TP; Laboratory of Immunopathology, Faculty of Medical Sciences, State University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro 20550-900, Brazil., Moraes MO; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Sarno EN; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Schmitz V; Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil; veronicaschmitz@ioc.fiocruz.br.
Jazyk:	angličtina
Zdroj:	ImmunoHorizons [Immunohorizons] 2020 Feb 07; Vol. 4 (2), pp. 47-56. Date of Electronic Publication: 2020 Feb 07.
DOI:	10.4049/immunohorizons.1900088
Abstrakt:	Erythema nodosum leprosum (ENL) is an inflammatory complication in leprosy. Yet, the involvement of ENL neutrophils in the inflammatory response against Mycobacterium leprae remains poorly explored. Our primary aim was to investigate the utility of the surface expression of neutrophil IL-10R1 as an ENL biomarker and, secondarily, to evaluate whether leprosy or healthy M. leprae -stimulated neutrophils produce cytokines and are able to respond to IL-10. We, in this study, describe a subpopulation of circulating neutrophils of ENL patients that exclusively expressed IL-10R1, providing evidence that IL-10R1 + neutrophils are present in ENL lesions. It was also found that ENL neutrophils, but not those of nonreactional leprosy controls, were able to secret detectable levels of TNF ex vivo and the addition of IL-10 blocked TNF release. It was likewise observed that M. leprae- stimulated, healthy neutrophils expressed IL-10R1 in vitro, and ENL-linked cytokines were released by M. leprae -cultured neutrophils in vitro. Moreover, consistent with the presence of a fully functional IL-10R, the addition of IL-10 prevented the release of M. leprae -induced cytokines. Most importantly, dead M. leprae revealed its superior capacity to induce CCL4 and IL-8 in primary neutrophils over live Mycobacterium , suggesting that M. leprae may hamper the inflammatory machinery as an immune escape mechanism. (Copyright © 2020 The Authors.)
Databáze:	MEDLINE
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