Photobiomodulation guided healing in a sub-critical bone defect in calvarias of rats.

Autor:	Magri AMP; Federal University of São Paulo (UNIFESP). Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil.; University Center of the Guaxupé Educational Foundation (UNIFEG), Avenida Dona Floriana, 463, Guaxupé, MG, 37800000, Brazil., Fernandes KR; Federal University of São Paulo (UNIFESP). Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil., Kido HW; Federal University of São Paulo (UNIFESP). Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil., Fernandes GS; Federal University of São Paulo (UNIFESP). Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil., Fermino SS; Federal University of São Paulo (UNIFESP). Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil., Gabbai-Armelin PR; Federal University of São Paulo (UNIFESP). Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil., Braga FJC; Consulmat LTDA, Rua Juan Lopes, 159, São Carlos, SP, 13567-020, Brazil., de Góes CP; Federal University of São Paulo (UNIFESP). Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil., Prado JLDS; Federal University of São Paulo (UNIFESP). Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil., Granito RN; Federal University of São Paulo (UNIFESP). Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil., Rennó ACM; Federal University of São Paulo (UNIFESP). Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil.
Jazyk:	angličtina
Zdroj:	Laser therapy [Laser Ther] 2019 Sep 30; Vol. 28 (3), pp. 171-179.
DOI:	10.5978/islsm.28_19-OR-13
Abstrakt:	Background: Photobiomodulation presents stimulatory effects on tissue metabolism, constituting a promising strategy to produce bone tissue healing. Objective: the aim of the present study was to investigate the in vivo performance of PBM using an experimental model of cranial bone defect in rats. Material and Methods: rats were distributed in 2 different groups (control group and PBM group). After the surgical procedure to induce cranial bone defects, PBM treatment initiated using a 808 nm laser (100 mW, 30 J/cm 2 , 3 times/week). After 2 and 6 weeks, animals were euthanized and the samples were retrieved for the histopathological, histomorphometric, picrosirius red staining and immunohistochemistry analysis. Results: Histology analysis demonstrated that for PBM most of the bone defect was filled with newly formed bone (with a more mature aspect when compared to CG). Histomorphomeric analysis also demonstrated a higher amount of newly formed bone deposition in the irradiated animals, 2 weeks post-surgery. Furthermore, there was a more intense deposition of collagen for PBM, with ticker fibers. Results from Runx-2 immunohistochemistry demonstrated that a higher immunostaining for CG 2 week's post-surgery and no other difference was observed for Rank-L immunostaining. Conclusion: This current study concluded that the use of PBM was effective in stimulating newly formed bone and collagen fiber deposition in the sub-critical bone defect, being a promising strategy for bone tissue engineering. (2019, Japan Medical Laser Laboratory.)
Databáze:	MEDLINE
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