Epigenomics of being bullied: changes in DNA methylation following bullying exposure.

Autor: Mulder RH; Institute of Education and Child Studies, Leiden University , Leiden, The Netherlands.; Department of Child and Adolescent Psychiatry/Psychology, Erasmus MC, University Medical Center Rotterdam , Rotterdam, The Netherlands.; Generation R Study Group, Erasmus MC, University Medical Center Rotterdam , Rotterdam, The Netherlands., Walton E; MRC Integrative Epidemiology Unit, Population Health Sciences, Bristol Medical School, University of Bristol , Bristol, UK.; Department of Psychology, University of Bath , Bath, UK., Neumann A; Department of Child and Adolescent Psychiatry/Psychology, Erasmus MC, University Medical Center Rotterdam , Rotterdam, The Netherlands.; Generation R Study Group, Erasmus MC, University Medical Center Rotterdam , Rotterdam, The Netherlands.; Lady Davis Institute for Medical Research, Jewish General Hospital , Montreal, Qc, Canada., Houtepen LC; MRC Integrative Epidemiology Unit, Population Health Sciences, Bristol Medical School, University of Bristol , Bristol, UK., Felix JF; Generation R Study Group, Erasmus MC, University Medical Center Rotterdam , Rotterdam, The Netherlands.; Department of Pediatrics, Erasmus MC, University Medical Center Rotterdam , Rotterdam, The Netherlands.; Department of Epidemiology, Erasmus MC, University Medical Center Rotterdam , Rotterdam, The Netherlands., Bakermans-Kranenburg MJ; Clinical Child & Family Studies, Vrije Universiteit Amsterdam , Amsterdam, The Netherlands., Suderman M; MRC Integrative Epidemiology Unit, Population Health Sciences, Bristol Medical School, University of Bristol , Bristol, UK., Tiemeier H; Department of Child and Adolescent Psychiatry/Psychology, Erasmus MC, University Medical Center Rotterdam , Rotterdam, The Netherlands.; Department of Social and Behavioral Science, Harvard TH Chan School of Public Health , Boston, MA, USA., van IJzendoorn MH; Department of Psychology, Education and Child Studies, Erasmus University Rotterdam , Rotterdam, The Netherlands.; School of Clinical Medicine, University of Cambridge , Cambridge, UK., Relton CL; MRC Integrative Epidemiology Unit, Population Health Sciences, Bristol Medical School, University of Bristol , Bristol, UK., Cecil CAM; Department of Child and Adolescent Psychiatry/Psychology, Erasmus MC, University Medical Center Rotterdam , Rotterdam, The Netherlands.; Department of Epidemiology, Erasmus MC, University Medical Center Rotterdam , Rotterdam, The Netherlands.; Department of Psychology, Institute of Psychology, Psychiatry & Neuroscience, King's College London , London, UK.
Jazyk: angličtina
Zdroj: Epigenetics [Epigenetics] 2020 Jun - Jul; Vol. 15 (6-7), pp. 750-764. Date of Electronic Publication: 2020 Jan 28.
DOI: 10.1080/15592294.2020.1719303
Abstrakt: Bullying among children is ubiquitous and associated with pervasive mental health problems. However, little is known about the biological pathways that change after exposure to bullying. Epigenome-wide changes in DNA methylation in peripheral blood were studied from pre- to post measurement of bullying exposure, in a longitudinal study of the population-based Generation R Study and Avon Longitudinal Study of Parents and Children (combined n = 1,352). Linear mixed-model results were meta-analysed to estimate how DNA methylation changed as a function of exposure to bullying. Sensitivity analyses including co-occurring child characteristics and risks were performed, as well as a Gene Ontology analysis. A candidate follow-up was employed for CpG (cytosine-phosphate-guanine) sites annotated to 5-HTT and NR3C1 . One site, cg17312179, showed small changes in DNA methylation associated to bullying exposure ( b = -2.67e-03, SE = 4.97e-04, p = 7.17e-08). This site is annotated to RAB14 , an oncogene related to Golgi apparatus functioning, and its methylation levels decreased for exposed but increased for non-exposed. This result was consistent across sensitivity analyses. Enriched Gene Ontology pathways for differentially methylated sites included cardiac function and neurodevelopmental processes. Top CpG sites tended to have overall low levels of DNA methylation, decreasing in exposed, increasing in non-exposed individuals. There were no gene-wide corrected findings for 5-HTT and NR3C1 . This is the first study to identify changes in DNA methylation associated with bullying exposure at the epigenome-wide significance level. Consistent with other population-based studies, we do not find evidence for strong associations between bullying exposure and DNA methylation.
Databáze: MEDLINE