Long-term development of human iPSC-derived pyramidal neurons quantified after transplantation into the neonatal mouse cortex.

Autor: D'Alessio R; Institut Pasteur, Neurobiologie Intégrative des Systèmes Cholinergiques, Département de Neuroscience, CNRS UMR 3571, 25 rue du Dr Roux, 75724, Paris Cedex 15, France., Koukouli F; Institut Pasteur, Neurobiologie Intégrative des Systèmes Cholinergiques, Département de Neuroscience, CNRS UMR 3571, 25 rue du Dr Roux, 75724, Paris Cedex 15, France; Sorbonne Université, Collège Doctoral, 75005, Paris, France., Blanchard S; Institut Pasteur, Neurobiologie Intégrative des Systèmes Cholinergiques, Département de Neuroscience, CNRS UMR 3571, 25 rue du Dr Roux, 75724, Paris Cedex 15, France., Catteau J; Institut Pasteur, Neurobiologie Intégrative des Systèmes Cholinergiques, Département de Neuroscience, CNRS UMR 3571, 25 rue du Dr Roux, 75724, Paris Cedex 15, France., Raïs C; Institut Pasteur, Neurobiologie Intégrative des Systèmes Cholinergiques, Département de Neuroscience, CNRS UMR 3571, 25 rue du Dr Roux, 75724, Paris Cedex 15, France; Sorbonne Université, Collège Doctoral, 75005, Paris, France., Lemonnier T; Institut du Fer à Moulin, Sorbonne Université, INSERM UMR-S 1270, 75005, Paris, France., Féraud O; INSERM UMR 935, ESTeam Paris Sud, SFR André Lwoff, Université Paris Sud, Villejuif, France; Infrastructure Nationale INGESTEM, Université Paris Sud, INSERM, Paris, France., Bennaceur-Griscelli A; INSERM UMR 935, ESTeam Paris Sud, SFR André Lwoff, Université Paris Sud, Villejuif, France; Infrastructure Nationale INGESTEM, Université Paris Sud, INSERM, Paris, France., Groszer M; Institut du Fer à Moulin, Sorbonne Université, INSERM UMR-S 1270, 75005, Paris, France., Maskos U; Institut Pasteur, Neurobiologie Intégrative des Systèmes Cholinergiques, Département de Neuroscience, CNRS UMR 3571, 25 rue du Dr Roux, 75724, Paris Cedex 15, France. Electronic address: umaskos@pasteur.fr.
Jazyk: angličtina
Zdroj: Developmental biology [Dev Biol] 2020 May 01; Vol. 461 (1), pp. 86-95. Date of Electronic Publication: 2020 Jan 23.
DOI: 10.1016/j.ydbio.2020.01.009
Abstrakt: One of the main obstacles for studying the molecular and cellular mechanisms underlying human neurodevelopment in vivo is the scarcity of experimental models. The discovery that neurons can be generated from human induced pluripotent stem cells (hiPSCs) paves the way for novel approaches that are stem cell-based. Here, we developed a technique to follow the development of transplanted hiPSC-derived neuronal precursors in the cortex of mice over time. Using post-mortem immunohistochemistry we quantified the differentiation and maturation of dendritic patterns of the human neurons over a total of six months. In addition, entirely hiPSC-derived neuronal parenchyma was followed over eight months using two-photon in vivo imaging through a cranial window. We found that transplanted hiPSC-derived neuronal precursors exhibit a "protracted" human developmental programme in different cortical areas. This offers novel possibilities for the sequential in vivo study of human cortical development and its alteration, followed in "real time".
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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