Large-Volume Vascularized Muscle Grafts Engineered From Groin Adipose Tissue in Perfusion Bioreactor Culture.

Autor:	An Y; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany.; Department of Plastic Surgery, Peking University Third Hospital, Beijing, China., Reimers K; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany., Allmeling C; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany., Liu J; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany., Lazaridis A; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany., Strauss S; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany., Vogt PM; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany.
Jazyk:	angličtina
Zdroj:	The Journal of craniofacial surgery [J Craniofac Surg] 2020 Mar/Apr; Vol. 31 (2), pp. 588-593.
DOI:	10.1097/SCS.0000000000006257
Abstrakt:	Background: Muscle tissue engineering still remains a major challenge. An axial vascular pedicle and a perfusion bioreactor are necessary for the development and maintenance of a large-volume engineered muscle tissue to provide circulation within the construct. This study aimed to determine whether large-volume vascularized muscle-like constructs could be made from rat groin adipose tissue in a perfusion bioreactor. Methods: Epigastric adipofascial flaps based on the inferior superficial epigastric vessels were elevated bilaterally in male Lewis rats and connected to the bioreactor. The system was run using a cable pump and filled with myogenic differentiation medium in the perfusion bioreactor for 1, 3, 5, or 7 weeks. The resulting tissue constructs were characterized with respect to the morphology and muscle-related expression of genes and proteins. Results: The histological examination demonstrated intact muscle-like tissue fibers; myogenesis was verified by the expression of myosin, MADS box transcription enhancer factor 2 D, desmin-a disintegrin and metalloproteinase domain (ADAM) 12-and M-cadherin using reverse transcription-polymerase chain reaction. Western blot analysis for desmin, MyoD1, N-cadherin, and ADAM12 was performed to verify the myogenic phenotype of the extracted differentiated tissue and prove the formation of muscle-like constructs. Conclusions: A large-volume vascularized muscle tissue could be engineered in a perfusion bioreactor. The resulting tissue had muscle-like histological features and expressed muscle-related genes and proteins, indicating that the trans-differentiation of adipose tissue into muscle tissue occurred.
Databáze:	MEDLINE
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